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. 2021 Oct;22(5):813-821.
doi: 10.1007/s40368-021-00626-1. Epub 2021 Jun 1.

Adhesion of three types of fissure sealant in saliva-contaminated and noncontaminated conditions: an in vitro study

Mahtab Memarpour  1 , Azade Rafiee  2 , Fereshteh Shafiei  3 , Tayebeh Dorudizadeh  4 , Sahba Kamran  4
Affiliations

Adhesion of three types of fissure sealant in saliva-contaminated and noncontaminated conditions: an in vitro study

Mahtab Memarpour et al. Eur Arch Paediatr Dent. 2021 Oct.

Abstract

Purpose: To evaluate the proportion of microleakage (PM), shear bond strength (SBS), and the fissure sealant (FS) interface by scanning electron microscopy (SEM) in three kinds of FS when the enamel surfaces were contaminated with saliva.

Methods: 198 sound third molar teeth were randomly divided into three pretreatment condition groups (n = 66): dry, saliva contamination removed by cotton pellet, or saliva removed by air-drying. A resin-based FS (Clinpro™), amorphous calcium phosphate-containing FS (Aegis®), or glass ionomer-based FS (Fuji Triage®) was applied on the treated enamel, and PM and SBS were assessed. Two specimens from each group were observed with SEM. p values < 0.05 was considered statistically significant.

Results: Glass ionomer-based FS showed the highest PM in all three surface conditions (p values < 0.05 were considered statistically significant). No significant difference in PM was observed between resin-based FS and amorphous calcium phosphate-containing FS (p > 0.05). Resin-based FS showed significantly greater SBS in all three surface conditions compared to glass ionomer-based FS. SEM observations showed that saliva contamination led to gaps at the enamel-sealant interface.

Conclusion: Neither cotton pellet-drying nor air-drying effectively removed saliva from the contaminated enamel surface. Glass ionomer-based FS showed the highest PM and the lowest SBS in contaminated and noncontaminated conditions. The highest SBS was obtained with resin-based FS.

Keywords: Enamel; Fissure sealant; Microleakage; Shear bond strength.

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References

    1. Al-Jobair A. Scanning electron microscope analysis of sealant penetration and adaptation in contaminated fissures. J Indian Soc Pedod Prev Dent. 2013;31:169–74. - DOI
    1. Alsaffar A, Tantbirojn D, Versluis A, Beiraghi S. Protective effect of pit and fissure sealants on demineralization of adjacent enamel. Pediatr Dent. 2011;33:491–5.
    1. Antonson SA, Antonson DE, Brener S, Crutchfield J, Larumbe J, Michaud C, Yazici AR, Hardigan PC, Alempour S, Evans D, Ocanto R. Twenty-four month clinical evaluation of fissure sealants on partially erupted permanent first molars: glass ionomer versus resin-based sealant. J Am Dent Assoc. 2012;143:115–22. - DOI
    1. Ashwin R, Arathi R. Comparative evaluation for microleakage between Fuji-VII glass ionomer cement and light-cured unfilled resin: a combined in vivo in vitro study. J Indian Soc Pedod Prev Dent. 2007;25:86–7. - DOI
    1. Asselin ME, Fortin D, Sitbon Y, Rompre PH. Marginal microleakage of a sealant applied to permanent enamel: evaluation of 3 application protocols. Pediatr Dent. 2008;30:29–33. - PubMed

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