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. 2021 Aug:187:106257.
doi: 10.1016/j.mimet.2021.106257. Epub 2021 May 29.

Rapid preparation of gastrointestinal nematode eggs from faeces for PCR identification

Nicole Hedley  1 , D Grant Richards  2 , Enzo A Palombo  3
Affiliations

Rapid preparation of gastrointestinal nematode eggs from faeces for PCR identification

Nicole Hedley et al. J Microbiol Methods. 2021 Aug.

Abstract

Detection of gastrointestinal nematodes (GIN) as both a qualitative and quantitative test is highly desirable. Methods such as multiplex and qPCR are capable of providing such results, but can be laborious and expensive. This paper presents a rapid, low-cost method of preparing GIN egg from faecal samples that produces DNA suitable for PCR analysis. We also describe a set of primers that are suitable for single-tube multiplex PCR.

Keywords: Gastrointestinal nematodes; Multiplex; Polymerase Chain Reaction; Primers.

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