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. 2021 May 1;7(5):356.
doi: 10.3390/jof7050356.

Comparison of Two Commercial Colorimetric Broth Microdilution Tests for Candida Susceptibility Testing: Sensititre YeastOne versus MICRONAUT-AM

Sophie Philips  1   2 , Frederik Van Hoecke  1 , Emmanuel De Laere  1 , Steven Vervaeke  1 , Roos De Smedt  1 , Jerina Boelens  3 , Deborah De Geyter  2 , Denis Piérard  2 , Katrien Lagrou  4
Affiliations

Comparison of Two Commercial Colorimetric Broth Microdilution Tests for Candida Susceptibility Testing: Sensititre YeastOne versus MICRONAUT-AM

Sophie Philips et al. J Fungi (Basel). .

Abstract

Two colorimetric broth microdilution antifungal susceptibility tests were compared, Sensititre YeastOne and MICRONAUT-AM for nine antifungal agents. One hundred clinical Candida isolates were tested, representing a realistic population for susceptibility testing in daily practice. The reproducibility characteristics were comparable. Only for fluconazole, caspofungin, 5-flucytosine and amphotericin B, an essential agreement of ≥90% could be demonstrated. Sensititre minimal inhibitory concentrations (MICs) were systematically higher than MICRONAUT MICs for all antifungals, except for itraconazole. CLSI clinical breakpoints (CBPs) and epidemiological cut-off values (ECVs) were used for Sensititre MICs while for MICRONAUT the EUCAST CBPs and ECVs were used. Only fluconazole, micafungin, and amphotericin B had a categorical agreement of ≥90%. For fluconazole, micafungin, and amphotericin B the susceptibility proportions were comparable. Susceptibility proportion of posaconazole and voriconazole was higher using the MICRONAUT system. For itraconazole and anidulafungin, the susceptibility proportion was higher using Sensititre. It was not possible to determine the true MIC values or the correctness of a S/I/R result since both commercial systems were validated against a different reference method. These findings show that there is a significant variability in susceptibility pattern and consequently on use of antifungals in daily practice, depending on the choice of commercial system.

Keywords: CLSI; Candida; EUCAST; MIC; MICRONAUT-AM; Sensititre YeastOne; antifungal agents; antifungal susceptibility testing; colorimetry.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
MIC values obtained with SY versus MIC values obtained with M-AM for each antifungal agent. The light colored dots represent MIC values within two dilutions between both methods. The dark colored dots represent MIC discrepancies of more than two dilutions.
Figure 1
Figure 1
MIC values obtained with SY versus MIC values obtained with M-AM for each antifungal agent. The light colored dots represent MIC values within two dilutions between both methods. The dark colored dots represent MIC discrepancies of more than two dilutions.
Figure 1
Figure 1
MIC values obtained with SY versus MIC values obtained with M-AM for each antifungal agent. The light colored dots represent MIC values within two dilutions between both methods. The dark colored dots represent MIC discrepancies of more than two dilutions.
Figure 2
Figure 2
Comparison of SY versus M-AM: percentage of S/WT and I/SDD Candida isolates for each antifungal agent.
See this image and copyright information in PMC

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