miRNA Profiling in the Chicken Liver under the Influence of Early Microbiota Stimulation with Probiotic, Prebiotic, and Synbiotic

Abstract

Epigenetic regulation of gene expression is a form of interaction of the external environment on reading and transcription of genetic information encoded in nucleic acids. We provided evidence that early stimulation of the chicken microbiota with in ovo delivered synbiotics influenced gene expression and DNA methylation in the liver. Therefore, we hypothesize that the stimulation of microbiota by administering bioactive substances in ovo also affects the activity of miRNA in liver. For the analysis of miRNA activity, RNA was isolated from liver of adult broiler chicken and native chicken breed. The animals received a prebiotic, probiotic and synbiotic in ovo on day 12 of egg incubation. The analysis of miRNA expression was performed using the LNA method on a miRNA panel selected on the basis of previous microarray experiments. We have found increased miRNA expression activity after probiotic and synbiotic administration, especially in native chicken breed. Our results suggest that prebiotics reduce or do not affect miRNA activity. We have also shown that miRNA activity is regulated by the substance and genotype of the chicken. We can conclude that miRNAs constitute an important component of the molecular mechanism of host-probiotic interaction in liver.

Keywords: bioactive substances; host-probiotic interaction; in ovo technology; intestinal microbiota.

Figure 1

Experimental setup (created with BioRender.com, https://biorender.com/, accessed on 30 April 2021).

Figure 2

Activity of miRNA in (A) broiler chicken (Ross 308) and (B) native chicken breed (Green-legged Partridgelike) after in ovo administration of probiotic prebiotic, and synbiotic based on average Ct value. The control is the group that received saline in ovo. The significance of changes in the miRNA activity was analyzed by t-test (p < 0.05) and labeled with (*).