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. 2021 May 6;26(9):2738.
doi: 10.3390/molecules26092738.

Peroxidase-Like Platinum Clusters Synthesized by Ganoderma lucidum Polysaccharide for Sensitively Colorimetric Detection of Dopamine

Xiang Lai  1 , Yu Han  1 , Jie Zhang  1 , Jinyu Zhang  1 , Weifeng Lin  2 , Zhiwei Liu  1 , Longgang Wang  1
Affiliations

Peroxidase-Like Platinum Clusters Synthesized by Ganoderma lucidum Polysaccharide for Sensitively Colorimetric Detection of Dopamine

Xiang Lai et al. Molecules. .

Abstract

The sensitive and selective detection of dopamine (DA) is very important for the early diagnosis of DA-related diseases. In this study, we reported the colorimetric detection of DA using Ganoderma lucidum polysaccharide (GLP) stabilized platinum nanoclusters (Ptn-GLP NCs). When Pt600-GLP NCs was added, 3,3',5,5'-tetramethylbenzidine (TMB) was rapidly catalyzed and oxidized to blue oxTMB, indicating the peroxidase-like activity of Pt600-GLP NCs. The catalytic reaction on the substrate TMB followed the Michaelis-Menton kinetics with the ping-pong mechanism. The mechanism of the colorimetric reaction was mainly due to the formation of hydroxyl radical (•OH). Furthermore, the catalytic reaction of Pt600-GLP NCs was used in the colorimetric detection of DA. The linear range for DA was 1-100 μM and the detection limit was 0.66 μM. The sensitive detection of DA using Pt-GLP NCs with peroxidase-like activity offers a simple and practical method that may have great potential applications in the biotechnology field.

Keywords: Ganoderma lucidum polysaccharide; colorimetric; detection; dopamine; platinum.

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Conflict of interest statement

All authors declare that they have no conflict of interest in this work.

Figures

Scheme 1
Scheme 1
The synthesis of Pt-GLP NCs and the schematic diagram of dopamine detection.
Figure 1
Figure 1
(a) GPC determination and (b) infrared spectrum of GLP.
Figure 2
Figure 2
(a) The protein content standard curve at 595 nm and (b) the reducing ability curve of GLP at 700 nm.
Figure 3
Figure 3
UV-Vis spectra of GLP, K2PtCl4, and Ptn-GLP NCs (n = 600, 1000, and 1400).
Figure 4
Figure 4
(a) HRTEM image and (b) histogram of Pt600-GLP NCs.
Figure 5
Figure 5
(a) The hydrodynamic size and (b) zeta potential of Ptn-GLP NCs in aqueous solution.
Figure 6
Figure 6
(a) UV-Vis spectra and (b) picture of the reaction product: (1) TMB + H2O2, (2) TMB + Pt600-GLP NCs, and (3) TMB + Pt600-GLP NCs + H2O2. Concentrations: TMB, 0.6 mM; Pt, 0.78 mM; H2O2, 0.3 M.
Figure 7
Figure 7
Effects of (a) temperature and (b) pH on the peroxidase-like property of Pt600-GLP NCs.
Figure 8
Figure 8
Steady-state kinetic assay and catalytic mechanism of Pt600-GLP NCs: (a) The H2O2 was 1.07 mM with varying TMB concentration. (b) The TMB was 0.17 mM with varying H2O2 concentration. (c,d) Double reciprocal plots of activity of Pt600-GLP NCs with the concentration of one substrate (H2O2 or TMB) fixed and the other varied.
Figure 9
Figure 9
(a) Fluorescence spectra and (b) histograms of fluorescence intensity of TA + H2O2 + Pt600-GLP NCs group and control groups after 12 h. The final concentrations of TA, H2O2, and Pt were 0.3 mM, 20 mM, and 49 μM, respectively.
Figure 10
Figure 10
(a) The absorption spectra and (b) absorbance at 652 nm of mixed solutions when Pt600-GLP NCs and TMB systems were added by various concentrations of DA (1–250 μM), (c) linear calibration chart of DA detection, and (d) the absorbance of dopamine (100 μM) and other interfering substances (1200 μM).
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