Study of Cytotoxic Properties of an Experimental Preparation with Features of a Dental Infiltrant

Abstract

Microinvasive dentistry is based on the treatment of early carious lesions with the use of dental infiltrants. The commercially available Icon dental infiltrant does not contain any bacteriostatic component. An experimental preparation enriched with the missing component was synthesised. The aim of this study was to evaluate the cytotoxicity of the experimental preparation. Mouse fibroblasts of the L-929 lineage were used for the in vitro study. Cell morphology and viability were assessed. In the cytotoxicity analysis, it was shown that the experimental preparation (42.8 ± 10.3) after 24 h at two-fold dilution showed similar cytotoxicity to Icon (42.7 ± 8.8) (p > 0.05), while at four-fold dilution experimental preparation (46.7 ± 3.1), it was less toxic than Icon (34.2 ± 3.1) (p < 0.05). The experimental preparation has the potential to provide an alternative to the Icon commercial preparation. Further research is needed to evaluate the cytotoxicity of the experimental preparation over a longer period of time.

Keywords: antimicrobial properties; dental materials; infiltrants; metronidazole; polymers.

Figure 1

Comparison of mean viability of L-929 cells (%) after 24 h of incubation with a 4-fold dilution of the extract of the experimental preparation and the Icon preparation (p < 0.05). * statistical significance.

Figure 2

(a) Plate with cultures of L-929 cells immediately after a 24 h incubation period with 2-fold diluted extracts of the test preparations. (b) MTT assay performed for cultures shown in image (a) (after formazan extraction with DMSO).

Figure 3

(a) L-929 line cells after 24 h incubation with a 2-fold diluted 24 h extract of the experimental preparation, (b) L-929 line cells after 24 h incubation with a 2-fold diluted 24 h extract of the experimental preparation after MTT assay, (c) L-929 cells after 24 h incubation with 2-fold diluted 24 h Icon extract, (d) L-929 cells after 24 h incubation with 2-fold diluted 24 h Icon extract after MTT test. (e) Control culture of adhered L-929 cells, (f) control culture of adhered L-929 cells after MTT test.

Figure 4

(Part 1) Scheme for obtaining 24 h extracts. (Part 2) Scheme for assessing cell viability.