Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 May 8;19(5):263.
doi: 10.3390/md19050263.

Biogenic Synthesis of Copper Nanoparticles Using Bacterial Strains Isolated from an Antarctic Consortium Associated to a Psychrophilic Marine Ciliate: Characterization and Potential Application as Antimicrobial Agents

Maria Sindhura John  1 , Joseph Amruthraj Nagoth  1 , Marco Zannotti  2 , Rita Giovannetti  2 , Alessio Mancini  1 , Kesava Priyan Ramasamy  1 , Cristina Miceli  1 , Sandra Pucciarelli  1
Affiliations

Biogenic Synthesis of Copper Nanoparticles Using Bacterial Strains Isolated from an Antarctic Consortium Associated to a Psychrophilic Marine Ciliate: Characterization and Potential Application as Antimicrobial Agents

Maria Sindhura John et al. Mar Drugs. .

Abstract

In the last decade, metal nanoparticles (NPs) have gained significant interest in the field of biotechnology due to their unique physiochemical properties and potential uses in a wide range of applications. Metal NP synthesis using microorganisms has emerged as an eco-friendly, clean, and viable strategy alternative to chemical and physical approaches. Herein, an original and efficient route for the microbial synthesis of copper NPs using bacterial strains newly isolated from an Antarctic consortium is described. UV-visible spectra of the NPs showed a maximum absorbance in the range of 380-385 nm. Transmission electron microscopy analysis showed that these NPs are all monodispersed, spherical in nature, and well segregated without any agglomeration and with an average size of 30 nm. X-ray powder diffraction showed a polycrystalline nature and face centered cubic lattice and revealed characteristic diffraction peaks indicating the formation of CuONPs. Fourier-transform infrared spectra confirmed the presence of capping proteins on the NP surface that act as stabilizers. All CuONPs manifested antimicrobial activity against various types of Gram-negative; Gram-positive bacteria; and fungi pathogen microorganisms including Escherichia coli, Staphylococcus aureus, and Candida albicans. The cost-effective and eco-friendly biosynthesis of these CuONPs make them particularly attractive in several application from nanotechnology to biomedical science.

Keywords: antibiotics; biomaterials; green synthesis; metal; nanotechnology.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
DLS of bio-CuNPs synthesized from the different bacterial strains: Marinomonas (MM), Rhodococcus (RH), Pseudomonas (PM), Brevundimonas (BM), and Bacillus (BC).
Figure 2
Figure 2
XRD profile of biosynthesized CuONPs. The obtained spectra reveal characteristic diffraction peaks indicating the formation of CuO in all bacterial NPs in comparison with simulation peaks (black line defined as the theoretical peaks).
Figure 3
Figure 3
TEM images of biosynthesized CuONPs from the five bacterial strains: Marinomonas ef1, Rhodococcus ef1, Pseudomonas ef1, Brevundimonas ef1, and Bacillus ef1.
Figure 4
Figure 4
Antibacterial activity of bio-CuONPs against pathogenic bacteria: Staphylococcus aureus (SA), Escherichia coli (EC), Klebsiella pneumoniae (KP), Pseudomonas aeruginosa (PA), Proteus mirabilis (PrM), Serratia marcescens (SM), Citrobacter koseri (CK), Acinetobacter baumanii (AB), Candida albicans (CA), and Candida parapsilosis (CP). Kirby–Bauer disk diffusion test. 1. MM-ef1, 2. RH-ef1, 3. BM-ef1, 4. PM-ef1, 5. BC-ef1, and C—control (1 mM of CuSO4).
See this image and copyright information in PMC

References

    1. Grigore M.E., Biscu E.R., Holban A.M., Gestal M.C., Grumezescu A.M. Methods of synthesis, properties and biomedical applications of CuO Nanoparticles. Pharmaceuticals. 2016;9:75. doi: 10.3390/ph9040075. - DOI - PMC - PubMed
    1. Katwal R., Kaur H., Sharma G., Naushad M., Pathania D. Electrochemical synthesized copper oxide Nanoparticles for enhanced photocatalytic and antimicrobial activity. J. Ind. Eng. Chem. 2015;31:173–184. doi: 10.1016/j.jiec.2015.06.021. - DOI
    1. Sastry M., Ahmad A., Khan M.I., Kumar R. Biosynthesis of metal Nanoparticles using fungi and actinomycetes. Curr. Sci. 2003;85:162–170.
    1. Mandal D., Bolander M.E., Mukhopadhyay D., Sarkar G., Mukherjee P. The use of microorganisms for the formation of metal nanoparticles and their application. Appl. Microbiol. Biotechnol. 2006;69:485–492. doi: 10.1007/s00253-005-0179-3. - DOI - PubMed
    1. Rahmatolahzadeh R., Aliabadi M., Motevalli K. Cu and CuO nanostructures: Facile hydrothermal synthesis, characterization and photocatalytic activity using new starting reagents. J. Mater. Sci. Mater. Electron. 2017;28:148–156. doi: 10.1007/s10854-016-5504-3. - DOI

MeSH terms

LinkOut - more resources