doi: 10.1021/jm00200a006.
Effect of sparsomycin analogues on the puromycin-peptidyl transferase reaction on ribosomes
- PMID: 340693
- DOI: 10.1021/jm00200a006
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Effect of sparsomycin analogues on the puromycin-peptidyl transferase reaction on ribosomes
J Med Chem.
1978 Feb.
Abstract
Sparsomycin analogues in which the unique -S(O)CH2SCH3 moiety was replaced by a variety of more easily accessible side chains were evaluated as inhibitors of the peptidyl transferase reaction with bacterial ribosomes. Competitive inhibition of acetyl[14C]phenylalanylpuromycin formation revealed that the sulfur-containing side chain of sparsomycin could be replaced with hydrophobic moieties, whereas complete removal of the -S(O)CH2SCH3 side chain eliminated the ribosomal binding affinity of sparsomycin. The specificity for the D isomer of S-deoxo-S-propylsparsomycin has established that the chiral carbon of sparsomycin analogues must be identical with the chirality of D-cysteinol for ribosomal binding.
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