Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 May 30;10(6):674.
doi: 10.3390/pathogens10060674.

Prevalence and Genetic Diversity of Group A Rotavirus Genotypes in Moscow (2019-2020)

Anton Yuzhakov  1   2 , Ksenia Yuzhakova  1 , Nadezhda Kulikova  1 , Lidia Kisteneva  1 , Stanislav Cherepushkin  1 , Svetlana Smetanina  3 , Marina Bazarova  3 , Anton Syroeshkin  4 , Tatiana Grebennikova  1
Affiliations

Prevalence and Genetic Diversity of Group A Rotavirus Genotypes in Moscow (2019-2020)

Anton Yuzhakov et al. Pathogens. .

Abstract

Group A rotavirus (RVA) infection is the leading cause of hospitalization of children under 5 years old, presenting with symptoms of acute gastroenteritis. The aim of our study was to explore the genetic diversity of RVA among patients admitted to Moscow Infectious Disease Clinical Hospital No. 1 with symptoms of acute gastroenteritis. A total of 653 samples were collected from May 2019 through March 2020. Out of them, 135 (20.67%) fecal samples were found to be positive for rotavirus antigen by ELISA. RT-PCR detected rotavirus RNA in 80 samples. Seven G-genotypes (G1, G2, G3, G4, G8, G9, and G12) and three P-genotypes (P[8], P[4], and P[6]) formed 9 different combinations. The most common combination was G9P[8]. However, for the first time in Moscow, the combination G3P[8] took second place. Moreover, all detected viruses of this combination belonged to Equine-like G3P[8] viruses that had never been detected in Russia before. The genotype G8P[8] and G9P[4] rotaviruses were also detected in Moscow for the first time. Among the studied rotaviruses, there were equal proportions of Wa and DS-1-like strains; previous studies showed that Wa-like strains accounted for the largest proportion of rotaviruses in Russia.

Keywords: DS-1-like; Wa-like; acute gastroenteritis; genotype; rotavirus A.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Distribution of acute gastroenteritis cases, rotavirus ELISA-positive samples and genotyped RVA among age groups.
Figure 2
Figure 2
Frequency of VP7 (a) and VP4 (b) genotypes in the samples.
Figure 3
Figure 3
Prevalence of G/P genotype combinations of group A rotavirus.
Figure 4
Figure 4
Phylogenetic tree of human rotavirus A strains based on partial VP7 nucleotide sequences. Bootstrap confidence limits are shown at each node; values less than 65 are not shown. Sequences from this study are marked by ●.
Figure 5
Figure 5
Phylogenetic tree of human rotavirus A strains based on partial VP4 nucleotide sequences. Bootstrap confidence limits are shown at each node; values less than 65 are not shown. Sequences from this study are marked by ●.
Figure 6
Figure 6
Phylogenetic tree of Russian genotype G3 human rotavirus A strains based on partial VP7 nucleotide sequences. Bootstrap confidence limits are shown at each node; values less than 65 are not shown. Sequences from this study are marked by ●, sequences from Moscow 2018–2019 are marked ○.
See this image and copyright information in PMC

References

    1. Crawford S.E., Ramani S., Tate J.E., Parashar U.D., Svensson L., Hagbom M., Franco M.A., Greenberg H.B., O’Ryan M., Kang G., et al. Rotavirus infection. Nat. Rev. Dis. Primers. 2017;3:17083. doi: 10.1038/nrdp.2017.83. - DOI - PMC - PubMed
    1. Tate J.E., Burton A.H., Boschi-Pinto C., Parashar U.D. World Health Organization-Coordinated Global Rotavirus Surveillance Network. Global, regional, and national estimates of rotavirus mortality in children <5 years of age, 2000–2013. Clin. Infect. Dis. 2016;62:S96–S105. - PMC - PubMed
    1. Report, State, 2019. On The State of Sanitary and Epidemiological Well-Being of the Population in the Russian Federation in 2018. M.: The Federal Service for Supervision of Consumer Rights Protection and Benefit Receiving a Person. [(accessed on 16 November 2020)]; Available online: https://www.rospotrebnadzor.ru/upload/iblock/798/gosudarstvennyy-doklad-.... (Report in Russian)
    1. Report, State, 2020. On the State of Sanitary and Epidemiological Well-Being of the Population in the Russian Federation in 2019. M.: The Federal Service for Supervision of Consumer Rights Protection and Benefit Receiving a Person. [(accessed on 16 November 2020)]; Available online: https://www.rospotrebnadzor.ru/upload/iblock/8e4/gosdoklad-za-2019_seb_2.... (Report in Russian)
    1. Raju B., Parikh R.P., Vetter V.V., Kolhapure S. Epidemiology of rotavirus gastroenteritis and need of high rotavirus vaccine coverage with early completion of vaccination schedule for protection against rotavirus diarrhea in India: A narrative review. Indian J. Public Health. 2019;63:243–250. doi: 10.4103/ijph.IJPH_307_18. - DOI - PubMed

LinkOut - more resources