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. 1988 Aug;165(2):489-98.
doi: 10.1016/0042-6822(88)90593-4.

In vitro replication and transcription of the segmented double-stranded RNA bacteriophage phi 6

M E Ewen  1 , H R Revel
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In vitro replication and transcription of the segmented double-stranded RNA bacteriophage phi 6

M E Ewen et al. Virology. 1988 Aug.

Erratum in

  • Virology 1988 Dec;167(2):661

Abstract

In vitro conditions that support viral-specific replication and transcription have been developed from Pseudomonas phaseolicola cells infected with the segmented double-stranded RNA bacteriophage phi 6. Transcription activity, previously shown to occur by semiconservative strand displacement, labeled (+) strands of all three genome segments and produced all three corresponding genome length messenger RNAs. Replication activity for each of the three double-stranded RNA segments is observed. Our criteria for replication were formation of genomic length double-stranded RNA products and at least (-) strand synthesis activity. Mn2+ and Sarkosyl together selectively inhibited transcription. Analysis of replication alone suggested that replication templates are the viral (+) messenger RNAs.

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