Review of microchip analytical methods for the determination of pathogenic Escherichia coli

Abstract

Bacterial infections remain the principal cause of mortality worldwide, making the detection of pathogenic bacteria highly important, especially Escherichia coli (E. coli). Current E. coli detection methods are labour-intensive, time-consuming, or require expensive instrumentation, making it critical to develop new strategies that are sensitive and specific. Microchips are an automated analytical technique used to analyse food based on their separation efficiency and low analyte consumption, which make them the preferred method to detect pathogenic bacteria. This review presents an overview of microchip-based analytical methods for analysing E. coli, which were published in recent years. Specifically, this review focuses on current research based on microchips for the detection of E. coli and reviews the limitations of microchip-based methods and future perspectives for the analysis of pathogenic bacteria.

Keywords: Aptamer; Catalysed hairpin assembly; Escherichia coli; Microchip; Pathogenic bacteria.