Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Nov;93(11):6267-6277.
doi: 10.1002/jmv.27121. Epub 2021 Jun 10.

The different replication between nonenveloped and quasi-enveloped hepatitis E virus

Hanbin Ji  1 , Shuangfeng Chen  1 , Qiuxia He  1 , Wenjing Wang  1 , Shilin Gong  1 , Zhongyao Qian  1 , Yike Zhang  1 , Daqiao Wei  1 , Wenhai Yu  2 , Fen Huang  1   3
Affiliations

The different replication between nonenveloped and quasi-enveloped hepatitis E virus

Hanbin Ji et al. J Med Virol. 2021 Nov.

Abstract

Hepatitis E virus (HEV) is the major pathogen of viral hepatitis. However, the understanding of the HEV life cycle is limited. In the present study, cells were separately infected with nonenveloped HEV (derived from feces or bile) or quasi-enveloped HEV (derived from the cell culture after serial passages, eHEV) and observed by confocal fluorescence microscopy to investigate the life cycle of HEV. HEV finished its binding and entry into host cells at first 6 h postinoculation (hpi). Cells inoculated with eHEV showed less infectivity than cells inoculated with nonenveloped HEV. Newly synthesized progeny virions were released into the supernatant of cell cultures from 48 hpi. Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot analysis results showed that the supernatant's progeny viruses were infectious even after five serial passages. These results show the significant difference between nonenveloped HEV and eHEV, which will provide novel insights into the HEV replication cycle. The efficient cell culture of HEV will promote the development of anti-HEV drugs and vaccines.

Keywords: binding and entry; nonenveloped HEV; quasi-enveloped HEV; replication cycle; viral particle release.

PubMed Disclaimer

References

REFERENCES

    1. Kamar N , Bendall R , Legrand-Abravanel F , et al. Hepatitis E. Lancet. 2012;379(9835):2477-2488. https://pubmed.ncbi.nlm.nih.gov/22549046/
    1. Rein DB , Stevens GA , Theaker J , Wittenborn JS , Wiersma ST . The global burden of hepatitis E virus genotypes 1 and 2 in 2005. Hepatology. 2012;55(4):988-997. https://pubmed.ncbi.nlm.nih.gov/22121109/
    1. Purcell RH , Emerson SU , Hepatitis E: an emerging awareness of an old disease. J Hepatol. 2008;48:494-503.
    1. Khuroo MS , Kamili S. Aetiology, clinical course and outcome of sporadic acute viral hepatitis in pregnancy. J Viral Hepat. 2003;10(1):61-69. https://pubmed.ncbi.nlm.nih.gov/12558914/
    1. Kumar A , Beniwal M , Kar P , Sharma JB , Murthy NS . Hepatitis E in pregnancy. Int J Gynaecol Obstet. 2004;85:240-244.

Publication types

LinkOut - more resources