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Comparative Study
. 1988 May 15;171(1):47-54.
doi: 10.1016/0003-2697(88)90123-6.

Colorimetric detection of the hydroxyl radical: comparison of the hydroxyl-radical-generating ability of various iron complexes

S Singh  1 , R C Hider
Affiliations
Comparative Study

Colorimetric detection of the hydroxyl radical: comparison of the hydroxyl-radical-generating ability of various iron complexes

S Singh et al. Anal Biochem. .

Abstract

An aromatic substrate for hydroxylation by OH radicals has been synthesized. Neither the substrate nor its hydroxylated product binds either iron(III) or iron(II). A spectrophotometric assay based on the hydroxylation of this substrate, N,N'-(5-nitro-1,3-phenylene)bisglutaramide, is described.

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