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Review
. 2021 May 17:8:553965.
doi: 10.3389/fmed.2021.553965. eCollection 2021.

Challenging Methicillin Resistance Detection in Bone and Joint Infections: Focus on the MRSA/SA SSTI® Strategy

Marie Titécat  1   2   3 , Caroline Loïez  1   3 , François Demaeght  3   4 , Jean-Thomas Leclerc  3   5   6 , Théo Martin  3   5 , Hervé Dezèque  3   5 , Henri Migaud  2   3   5 , Eric Senneville  2   3   4
Affiliations
Review

Challenging Methicillin Resistance Detection in Bone and Joint Infections: Focus on the MRSA/SA SSTI® Strategy

Marie Titécat et al. Front Med (Lausanne). .

Abstract

The genus Staphylococcus is the main causative agent of bone and joint infections (BJI) in which outcomes are impacted by both effective surgical and appropriate antimicrobial management. In this context, methicillin resistance (MR) detection is a microbiological challenge to optimize the anti-staphylococcal drug coverage and to secure the surgical procedure. During the last decade, molecular tools have been developed to rapidly detect bacterial-resistant strains in clinical samples. The GeneXpert MRSA/SA SSTI® assay (Cepheid, Sunnyvale, CA, USA) is a real-time PCR method aimed at detecting methicillin-resistant Staphylococcus aureus (MRSA) in skin and soft tissues infections. In the literature, this test has been reported to be diverted from its original purpose to be evaluated in surgical samples. Within the current review, we update the GeneXpert MRSA/SA SSTI® assay performance in staphylococcal species determination (i.e., S. aureus vs. coagulase-negative species) together with MR genotype detection, when performed in osteoarticular infections.

Keywords: PCR; Xpert MRSA/SA SSTI®; bone and joint infections; conventionnal culture; methicillin resistance.

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Conflict of interest statement

ES declares personal honoraria from Cepheid for speaker activity. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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