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. 2021:2277:433-447.
doi: 10.1007/978-1-0716-1270-5_27.

The Isolation and Deep Sequencing of Mitochondrial DNA

Alexander G Bury  1   2 , Fiona M Robertson  1   3 , Angela Pyle  1   3 , Brendan A I Payne  1   3 , Gavin Hudson  4   5
Affiliations

The Isolation and Deep Sequencing of Mitochondrial DNA

Alexander G Bury et al. Methods Mol Biol. 2021.

Abstract

In recent years, next-generation sequencing (NGS) has become a powerful tool for studying both inherited and somatic heteroplasmic mitochondrial DNA (mtDNA) variation. NGS has proved particularly powerful when combined with single-cell isolation techniques, allowing the investigation of low-level heteroplasmic variants both between cells and within tissues. Nevertheless, there remain significant challenges, especially around the selective enrichment of mtDNA from total cellular DNA and the avoidance of nuclear pseudogenes. This chapter summarizes the techniques needed to enrich, amplify, sequence, and analyse mtDNA using NGS .

Keywords: Massively parallel sequencing; Mitochondrial DNA; Mitochondrial isolation deep sequencing.

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