Detection of genetic variation and activity analysis of the promoter region of the cattle tRNA-modified gene TRDMT1

Abstract

The tRNA modification gene in eukaryotes is relatively conservative. As an important modification gene, the TRDMT1 gene plays an important role in maintaining tRNA structural maintenance and reducing mistranslation of protein translation by methylation of specific tRNA subpopulations. Mouse and zebrafish TRDMT1 knockout experiments indicate that it may mediate growth and development through tRNA modification. However, there are no systematic reports on the function of tRNA-modified genes in livestock. In this study, Qinchuan cattle DNA pool sequencing technology was used. A G $>$ C mutation in the $-1223$ bp position upstream of the TRDMT1 translation initiator codon was found. At this locus, the dual-luciferase assay indicated that different genotypes cause differences in transcriptional activity ( $P<0.05$ ). Our experiment detected a natural genetic variation of a tRNA modification gene TRDMT1, which may provide potential natural molecular materials for the study of tRNA modification.

Figure 1

TRDMT1 promoter genetic variation site.

Figure 2

Prediction result of the CpG island in the TRDMT1 promoter region.

Figure 3

TRANSFAC predicts differences in promoter variation and transcription factor binding.

Figure 4

Genomatix predicts differences in promoter variation and transcription factor binding.

Figure 5

Comparison of relative luciferase activity of TRDMT1 promoter variant.

Figure 6

Expression profiling of the TRDMT1 gene in Qinchuan cattle.