Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Jan;132(1):101-112.
doi: 10.1111/jam.15175. Epub 2021 Jul 6.

Motility, biofilm formation and associated gene expression in Vibrio parahaemolyticus impaired by co-culture with live Ulva fasciata

Yan Qiao  1 , Lijuan Feng  1 , Rong Jia  1 , Yuqin Luo  1 , Qiao Yang  1
Affiliations

Motility, biofilm formation and associated gene expression in Vibrio parahaemolyticus impaired by co-culture with live Ulva fasciata

Yan Qiao et al. J Appl Microbiol. 2022 Jan.

Abstract

Aims: Vibrio parahaemolyticus is one of the most frequently occurred pathogens in mariculture. This study aimed to explore the mechanism of the impact of Ulva fasciata on the motility and biofilm formation of V. parahaemolyticus.

Methods and results: The inhibitory effect of U. fasciata on a V. parahaemolyticus, isolated from clam maricultural sediment, was examined by co-culture of them. The live U. fasciata significantly inhibited the swimming behaviour, twitching behaviour and biofilm formation of V. parahaemolyticus JF, with inhibition rates range of 2.48%-20.26%, 1.59%-39.18% and 28.3%-94.7% under different nitrate and phosphate levels, respectively. The results of transcriptome sequencing showed that 210 significantly differentially expressed genes (DEGs) were found in strain JF between the presence and absence of U. fasciata, including 90 upregulated genes and 120 downregulated genes. According to GO (Gene Ontology) function enrichment and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis, the downregulated genes of JF were partially enriched in flagella assembly (fliC, fliK, fliG, fliN, fliH, fliI, fliJ and fliA), bacterial chemotaxis (mCP, cheB, cheW and cheY) and biofilm formation (fliA/σ28 and eps), which explained the suppressed motility and biofilm formation of V. parahaemolyticus JF under U. fasciata stress.

Conclusions: Live U. fasciata significantly impaired the motility and biofilm formation of V. parahaemolyticus, which could occur in niches with either sufficient or inadequate nutrient (nitrate and phosphate) concentrations. The DEGs of V. parahaemolyticus modulated by U. fasciata were enriched mainly in the flagellar assembly, bacterial chemotaxis and biofilm pathways.

Significance and impact of the study: New information on how V. parahaemolyticus respond to U. fasciata regarding motility and adhesion behaviours, and the mechanism of that was firstly explored in this study. The results suggested that the seaweed U. fasciata has promising prospects as an environmentally friendly preventive measure to treat vibriosis in mariculture.

Keywords: antimicrobials; biofilms; bioinformatics; genes; pathogenesis.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

REFERENCES

    1. Anaya-Rosas, R.E., Rivas-Vega, M.E., Miranda-Baeza, A., Piña-Valdez, P. & Nieves-Soto, M. (2019) Effects of a co-culture of marine algae and shrimp (Litopenaeus vannamei) on the growth, survival and immune response of shrimp infected with Vibrio parahaemolyticus and white spot virus (WSSV). Fish & Shellfish Immunology, 87, 136-143.
    1. Bentil, J.A., Thygesen, A., Lange, L., Mensah, M. & Meyer, A.S. (2019) Green seaweeds (Ulva fasciata sp.) as nitrogen source for fungal cellulase production. World Journal of Microbiology & Biotechnology, 35, 82.
    1. Berri, M., Slugocki, C., Olivier, M., Helloin, E., Jacques, I., Salmon, H. et al. (2016) Marine-sulfated polysaccharides extract of Ulva armoricana green algae exhibits an antimicrobial activity and stimulates cytokine expression by intestinal epithelial cells. Journal of Applied Phycology, 28, 2999-3008.
    1. Bownik, A. (2017) Daphnia swimming behaviour as a biomarker in toxicity assessment: a review. Science of the Total Environment, 601-602, 194-205.
    1. Bracken, M.E.S. & Williams, S.L. (2013) Realistic changes in seaweed biodiversity affect multiple ecosystem functions on a rocky shore. Ecology, 94, 1944-1954.