In vitro construction of plasmids which result in overproduction of the protein product of the araC gene of Escherichia coli

Abstract

Derivatives of the Escherichia coli drug resistance plasmid pMB-9 were constructed which contain the promoter from the lactose operon of E. coli fused to the araC gene of E. coli. E. coli possessing these plasmids contain about 50 times as much of the araC gene product as do cells with a wild-type araC gene and promotor.