Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 May;7(5):1395-402.
doi: 10.1002/j.1460-2075.1988.tb02956.x.

A direct link between core histone acetylation and transcriptionally active chromatin

T R Hebbes  1 , A W Thorne, C Crane-Robinson
Affiliations

A direct link between core histone acetylation and transcriptionally active chromatin

T R Hebbes et al. EMBO J. 1988 May.

Abstract

An antiserum raised against chemically acetylated histone H4 was found to recognize the epitope epsilon-N-acetyl lysine. Affinity-purified antibodies were used to fractionate oligo- and mononucleosomal chromatin fragments from the nuclei of 15-day chicken embryo erythrocytes. Antibody-bound chromatin was found to contain elevated levels of acetylated core histones. On probing with sequences of alpha D globin, an actively transcribed gene, the antibody-bound chromatin was 15- to 30-fold enriched relative to the input chromatin. Using ovalbumin sequences as a probe, no enrichment was observed. The results demonstrate directly that transcriptionally active genes carry acetylated core histones.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1966 Apr;55(4):805-12 - PubMed
    1. Proc Natl Acad Sci U S A. 1964 May;51:786-94 - PubMed
    1. J Biol Chem. 1970 Mar 25;245(6):1261-6 - PubMed
    1. J Cell Biol. 1973 Jun;57(3):773-81 - PubMed
    1. J Biol Chem. 1974 Jun 25;249(12):3960-72 - PubMed

Publication types