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Review
. 2021 Dec;18(12):1463-1476.
doi: 10.1038/s41592-021-01156-w. Epub 2021 Jun 7.

Best practices and tools for reporting reproducible fluorescence microscopy methods

Paula Montero Llopis  1 , Rebecca A Senft  2 , Tim J Ross-Elliott  3 , Ryan Stephansky  3 , Daniel P Keeley  4 , Preman Koshar  4 , Guillermo Marqués  5 , Ya-Sheng Gao  6 , Benjamin R Carlson  6 , Thomas Pengo  7 , Mark A Sanders  5 , Lisa A Cameron  6 , Michelle S Itano  4
Affiliations
Review

Best practices and tools for reporting reproducible fluorescence microscopy methods

Paula Montero Llopis et al. Nat Methods. 2021 Dec.

Abstract

Although fluorescence microscopy is ubiquitous in biomedical research, microscopy methods reporting is inconsistent and perhaps undervalued. We emphasize the importance of appropriate microscopy methods reporting and seek to educate researchers about how microscopy metadata impact data interpretation. We provide comprehensive guidelines and resources to enable accurate reporting for the most common fluorescence light microscopy modalities. We aim to improve microscopy reporting, thus improving the quality, rigor and reproducibility of image-based science.

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References

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