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. 1988 Aug 15;44(8):702-4.
doi: 10.1007/BF01941034.

The use of iodinated density gradient media for the isolation of rod outer segments

R A Delgadillo  1 , R M Smets, Q R Yang, D Vanden Berghe, A Neetens
Affiliations

The use of iodinated density gradient media for the isolation of rod outer segments

R A Delgadillo et al. Experientia. .

Abstract

Sucrose, nycodenz, metrizamide and a mixture of equal volumes of sucrose and metrizamide were used as density gradient media for the isolation of retinal rod outer segments. The high osmolarity of sucrose had a strongly negative effect on the nature of the rod outer segments, whereas they were much better preserved using iodinated density gradient media such as nycodenz and metrizamide for their isolation.

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