Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Jan;17(1):99-100.
doi: 10.4103/1673-5374.314309.

Small scale adeno-associated virus-vector production for preclinical gene delivery based on chloroform precipitation

Marcus Davidsson  1 , Andreas Heuer  2
Affiliations

Small scale adeno-associated virus-vector production for preclinical gene delivery based on chloroform precipitation

Marcus Davidsson et al. Neural Regen Res. 2022 Jan.
No abstract available

PubMed Disclaimer

Conflict of interest statement

None

Figures

Figure 1
Figure 1
AAV research and production. Top part of the figure shows the three main areas in AAV research. i) AAV production – the goal is to produce AAV batches of high quality and purity while keeping the process time and cost-effective. ii) AAV cassette – where the end goal is to find cell-specific enhancer-promoter combination as well as efficient termination signals. iii) AAV capsid – AAV development is mainly focused on finding new cell-specific capsids or capsids with unique properties. However, increased packaging capacity and evading immune responses are also of great interest to the field of gene therapy. Bottom part of the figure shows a colour scale from green (Chloroform) to purple (Iodixanol) and for which experiments each method is more suitable. AAV: Adeno-associated virus.
See this image and copyright information in PMC

References

    1. Aldrin-Kirk P, Heuer A, Rylander Ottosson D, Davidsson M, Mattsson B, Bjorklund T. Chemogenetic modulation of cholinergic interneurons reveals their regulating role on the direct and indirect output pathways from the striatum. Neurobiol Dis. 2018;109:148–162. - PubMed
    1. Blankvoort S, Descamps LAL, Kentros C. Enhancer-Driven Gene Expression (EDGE) enables the generation of cell type specific tools for the analysis of neural circuits. Neurosci Res. 2020;152:78–86. - PubMed
    1. Chen TW, Wardill TJ, Sun Y, Pulver SR, Renninger SL, Baohan A, Schreiter ER, Kerr RA, Orger MB, Jayaraman V, Looger LL, Svoboda K, Kim DS. Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature. 2013;499:295–300. - PMC - PubMed
    1. Chtarto A, Bender HU, Hanemann CO, Kemp T, Lehtonen E, Levivier M, Brotchi J, Velu T, Tenenbaum L. Tetracycline-inducible transgene expression mediated by a single AAV vector. Gene Ther. 2003;10:84–94. - PubMed
    1. Davidsson M, Negrini M, Hauser S, Svanbergsson A, Lockowandt M, Tomasello G, Manfredsson FP, Heuer A. A comparison of AAV-vector production methods for gene therapy and preclinical assessment. Sci Rep. 2020;10:21532. - PMC - PubMed