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. 1988 Aug 29;236(2):352-6.
doi: 10.1016/0014-5793(88)80054-1.

Cloning and complete amino acid sequences of human and murine basement membrane protein BM-40 (SPARC, osteonectin)

B Lankat-Buttgereit  1 , K Mann, R Deutzmann, R Timpl, T Krieg
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Free article

Cloning and complete amino acid sequences of human and murine basement membrane protein BM-40 (SPARC, osteonectin)

B Lankat-Buttgereit et al. FEBS Lett. .
Free article

Abstract

Amino acid sequences of 285 and 286 residues, respectively, were deduced for mouse and human BM-40 from cDNA clones isolated from expression libraries. The sequences showed 92% identity and were also essentially identical to those of bone osteonectin and of the parietal endoderm protein SPARC. About 60% of the mouse BM-40 sequence was confirmed by Edman degradation. Two of the seven disulfide bonds were localized which apparently separate two distinct domains of mouse BM-40.

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