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. 1977 Dec;4(12):4063-75.
doi: 10.1093/nar/4.12.4063.

Detection of nucleoside Q precursor in methyl-deficient E.coli tRNA

N Okada, T Yasuda, S Nishimura
Free PMC article

Detection of nucleoside Q precursor in methyl-deficient E.coli tRNA

N Okada et al. Nucleic Acids Res. 1977 Dec.
Free PMC article

Abstract

32P-Labeled tRNAAsn was isolated from methyl-deficient E. coli tRNA. Nucleotide sequence analysis showed that tRNAAsn contains three derivatives of the Q nucleoside, possibly Q precursors, in addition to guanosine in the first position of the anticodon. One of the Q precursors was isolated on a large scale. Its UV spectra were identical with those of normal Q, indicating that 7-deazaguanosine structure having a side chain at position C-7 is complete in the Q precursor. No radioactivity was incorporated into Q or Q precursors from either [methyl-14C]methionine, [1-14C]methionine or [U-14C]methionine, showing that methionine was not directly involved in the formation of Q.

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References

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