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. 2021 Jun 11;100(23):e26221.
doi: 10.1097/MD.0000000000026221.

Vitamin K2 (Menaquinone-7) supplementation does not affect vitamin K-dependent coagulation factors activity in healthy individuals

Ruijun Ren  1 , Jia Liu  2 , Guo Cheng  2 , Jing Tan  2
Affiliations

Vitamin K2 (Menaquinone-7) supplementation does not affect vitamin K-dependent coagulation factors activity in healthy individuals

Ruijun Ren et al. Medicine (Baltimore). .

Abstract

Background: Vitamin K has long been regarded as a procoagulant drug by physicians, and concerns have been raised with regard to its effects on hemostasis. Although many studies have shown that vitamin K supplementation is safe for thrombotic events, the effect of vitamin K supplementation on the activities of vitamin K dependent procoagulation factors in healthy individuals is not available.

Objectives: This study aimed to investigate whether vitamin K2 supplementation at recommended doses affects the activity of vitamin K dependent procoagulation factors in healthy individuals without any anticoagulation treatment.

Design: Forty healthy volunteers between 25 and 40 years of age were recruited. Menaquinone-7 (MK-7) was administrated at 90 μg for 30 days. Prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), and blood coagulation factors II, VII, IX, and X activities and Protein induced by vitamin K absence or antagonist-II (PIVKA-II) were measured on days 0 and 30 after MK-7 administration.

Results: PT, APTT, and TT showed no significant differences on day 30 when compared with baseline. The activities of coagulation factors II, VII, IX, and X on day 30 showed no significant differences with those at baseline. PIVKA-II levels were unchanged after 30 days of MK-7 supplementation.

Conclusions: MK-7 supplementation at recommended dosage does not affect vitamin K-dependent coagulation factors' coagulation activity, and does not enhance the carboxylation of prothrombin in healthy individuals. This indicated that MK-7 administration does not alter hemostatic balance in healthy populations without anticoagulation treatment.

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Conflict of interest statement

The authors have no conflicts of interests to disclose.

Figures

Figure 1
Figure 1
APTT, PT, and TT were measured at baseline, day 7 and day 30 after MK-7 supplement, and did not show significant differences at each time points.
Figure 2
Figure 2
On day 0 and day30 of MK-7 administration, plasma was diluted at 1:10 ratio. Factor II (F II:C), factor VII (F VII:C), factor IX (F IX:C) and factor X (F X:C) activities showed similar decrement on day 0 and day 30.
Figure 3
Figure 3
In the forty healthy subjects, the uncarboxylated prothrombin (PIVKA-II) levels did not change with 30 days MK-7 supplementation (P = .19).
See this image and copyright information in PMC

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