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. 2021 Nov;30(9):1781-1788.
doi: 10.1007/s10646-021-02425-2. Epub 2021 Jun 11.

The distribution characteristics of β-propeller phytase genes in rhizosphere sediment provide insight into species specialty from phytic mineralization in subtropical and tropical seagrass ecosystems

Liyun Lin  1   2   3   4 , Juan Ling  5   6   7   8 , Qiuying Peng  1   2   3   9 , Xiancheng Lin  1   2   3   9 , Weiguo Zhou  1   2   10 , Yanying Zhang  11 , Qingsong Yang  1   2   10   9 , Manzoor Ahamad  1   2   3 , Ying Zhang  1   2   3   9 , Cong Wang  1   2   3 , Youshao Wang  1   2   10 , Junde Dong  12   13   14   15   16
Affiliations

The distribution characteristics of β-propeller phytase genes in rhizosphere sediment provide insight into species specialty from phytic mineralization in subtropical and tropical seagrass ecosystems

Liyun Lin et al. Ecotoxicology. 2021 Nov.

Abstract

Seagrass meadows have seriously deteriorated in recent years. Seagrass associated phytate-mineralizing rhizobacteria potentially have functions related to seagrass nutrition, health and sustainable growth. The β-propeller phytases (BPPs) are the only phytase family in aquatic environments, but there are few studies on the BPP community structure of seagrass. In this study, clone libraries and quantitative PCR (qPCR) assays were used to compare the diversity and abundances of the BPP communities of Halodule endl, Halophila ovalis and Thalassia hemprichii in Xisha and Sanya, and to investigate the distribution characteristics of BPP genes in the rhizosphere sediment, which provedes insight into species specialty from phytic mineralization in subtropical and tropical seagrass ecosystems. The highest diversity of BPP genes was found for Thalassia hemprichii in Sanya Bay. Thalassia hemprichii in Sanya had higher abundances of BPPs, which were linked to Gammaproteobacteria. The BPP community diversity and OTUs of Thalassia hemprichii in Sanya were much higher than those of Thalassia hemprichii on Yongxing Island and Stone Island. The seagrass BPP communities had higher diversity and evenness from sampling sites with more human activity. The qPCR results showed that the abundance of phytate acid-degradating bacteria was approximately three times larger in Thalassia hemprichii rhizosphere sediment samples than in Halodule endl and Halophila ovalis rhizosphere sediment samples. This study highlighted that the diversity and abundances of bacteria genetically encoding BPP in the rhizosphere of Thalassia hemprichii were clearly higher than those of Halodule endl and Halophila ovalis. Further study of microbial phosphorus cycling will provide new insights into seagrass meadow ecosystems.

Keywords: Abundance; Diversity; Rhizobacteria; Seagrass; β-propeller phytase.

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