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Review
. 2021 Dec:65:42-48.
doi: 10.1016/j.cbpa.2021.04.012. Epub 2021 Jun 10.

Caged lipids for subcellular manipulation

Scotland Farley  1 , Aurélien Laguerre  1 , Carsten Schultz  2
Affiliations
Review

Caged lipids for subcellular manipulation

Scotland Farley et al. Curr Opin Chem Biol. 2021 Dec.

Abstract

We present recently developed strategies to manipulate lipid levels in live cells by light. We focus on photoremovable protecting groups that lead to subcellular restricted localization and activation and discuss alternative techniques. We emphasize the development of organelle targeting of caged lipids and discuss recent advances in chromatic orthogonality of caging groups for future applications.

Keywords: Fluorescence; Lipid transport; Photoactivation; Signaling.

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Conflict of interest statement

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Figure 1.
Figure 1.
Example of photo-removable protecting groups expanding the spectral range of absorption for the uncaging of small molecules from UV to visible wavelengths.
Figure 2.
Figure 2.
A. Chemical structure of tri-functional phosphatidylinositol (PI). Accumulation of PI in endo membranes after uncaging for 5 sec (B) or 2 min (C) followed by 2 min of photo-crosslinking. The location of the lipid was monitored by confocal microscopy after fixation and labelling the terminal alkyne of the lipid tail with an azide-bearing fluorophore via copper click chemistry.
Figure 3.
Figure 3.
Chemical structures of photo-removable protecting groups targeting distinct cellular membranes. This allows local photo-release of signaling lipids in live cells. R refers to the lipid moiety.
See this image and copyright information in PMC

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