All-Optical Electrophysiology in hiPSC-Derived Neurons With Synthetic Voltage Sensors
- PMID: 34122014
- PMCID: PMC8193062
- DOI: 10.3389/fncel.2021.671549
All-Optical Electrophysiology in hiPSC-Derived Neurons With Synthetic Voltage Sensors
Abstract
Voltage imaging and "all-optical electrophysiology" in human induced pluripotent stem cell (hiPSC)-derived neurons have opened unprecedented opportunities for high-throughput phenotyping of activity in neurons possessing unique genetic backgrounds of individual patients. While prior all-optical electrophysiology studies relied on genetically encoded voltage indicators, here, we demonstrate an alternative protocol using a synthetic voltage sensor and genetically encoded optogenetic actuator that generate robust and reproducible results. We demonstrate the functionality of this method by measuring spontaneous and evoked activity in three independent hiPSC-derived neuronal cell lines with distinct genetic backgrounds.
Keywords: BeRST-1; optogenetics; phenotyping; stem cells; voltage imaging.
Copyright © 2021 Puppo, Sadegh, Trujillo, Thunemann, Campbell, Vandenberghe, Shan, Akkouh, Miller, Bloodgood, Silva, Dale, Einevoll, Djurovic, Andreassen, Muotri and Devor.
Conflict of interest statement
ARM is a co-founder and has equity interest in TISMOO, a company dedicated to genetic analysis and brain organoid modeling focusing on therapeutic applications customized for autism spectrum disorder and other neurological disorders with genetic origins. The terms of this arrangement have been reviewed and approved by the University of California San Diego in accordance with its conflict of interest policies. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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