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. 2021 Sep;38(9):2283-2289.
doi: 10.1007/s10815-021-02220-2. Epub 2021 Jun 14.

Oocyte stimulation parameters influence the number and proportion of mature oocytes retrieved in assisted reproductive technology cycles

Damla C Gonullu  1 , David H McCulloh  2 , LeRoy G Robinson Jr  3 , Cheongeun Oh  4 , David L Keefe  5   3
Affiliations

Oocyte stimulation parameters influence the number and proportion of mature oocytes retrieved in assisted reproductive technology cycles

Damla C Gonullu et al. J Assist Reprod Genet. 2021 Sep.

Abstract

Purpose: Whether differences in stimulation parameters alter the number and proportion of MII oocytes retrieved.

Methods: Records of 2546 patients were examined, looking at age, day 2/3 follicle-stimulating hormone (FSH) and estradiol (E2) levels, total dose of gonadotropins administered (including FSH and human menopausal gonadotropin [hMG]), fraction of hMG administered, number of days of treatment with gonadotropins, and the dose of gonadotropins administered per day. We segregated the patients into 3 different classes depending on the trigger method used and 2 groups based on egg freeze vs. ICSI. Multiple regression methods were used to examine associations between stimulation parameters and the total number of eggs, number of immature oocytes (Poisson regression), and the fraction of retrieved oocytes that were immature (Logistic regression).

Results: After adjustments for different triggers and egg freeze versus ICSI, both the #immature oocytes and the immature fraction of oocytes were associated with the total gonadotropin dose (inversely) and the gonadotropin dose/day (positively). Other parameters were associated with the number of immature oocytes but were also associated with the number of oocytes retrieved.

Conclusions: Stimulations using less total gonadotropin and more gonadotropin per day were associated with more immaturity. The type of trigger method used for final maturation was associated with immaturity but was believed to be predominantly due to trigger assignment to patients based on response. The association between use of ICSI and less immaturity was believed to be due to additional time for maturation in the ICSI group.

Keywords: Assisted reproductive technology; Oocyte maturation; Oocyte stimulation; Ovulation trigger.

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References

    1. Beall S, Brenner C, Segars J. Oocyte maturation failure: a syndrome of bad eggs. Fertil Steril. 2010;94(7):2507–2513. doi: 10.1016/j.fertnstert.2010.02.037. - DOI - PMC - PubMed
    1. Practice Committees of the American Society for Reproductive Medicine, the Society of Reproductive Biologists and Technologists, and the Society for Assisted Reproductive Technology Electronic address: jgoldstein@asrm.org. In vitro maturation: a committee opinion. Fertil Steril. 2021;115(2):298–304. doi: 10.1016/j.fertnstert.2020.11.018. - DOI - PubMed
    1. Brown HM, Dunning KR, Sutton-McDowall M, Gilchrist RB, Thompson JG, Russell DL. Failure to launch: aberrant cumulus gene expression during oocyte in vitro maturation. Reproduction. 2017;153(3):R109–R120. doi: 10.1530/REP-16-0426. - DOI - PubMed
    1. Check JH. Mild ovarian stimulation. J Assist Reprod Genet. 2007;24(12):621–627. doi: 10.1007/s10815-007-9179-9. - DOI - PMC - PubMed
    1. Lonergan P, Fair T. Maturation of Oocytes in Vitro. Annu Rev Anim Biosci. 2016;4:255–268. doi: 10.1146/annurev-animal-022114-110822. - DOI - PubMed

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