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. 1988 Jul;13(7):601-3.
doi: 10.1007/BF00973274.

A microelectrophoretic method for the evaluation of GABA transaminase activity

A Cupello  1 , S Larsson, A Palm, M V Rapallino
Affiliations

A microelectrophoretic method for the evaluation of GABA transaminase activity

A Cupello et al. Neurochem Res. 1988 Jul.

Abstract

The reduction to the micro-scale of a recently described electrophoretic method for the evaluation of GABA catabolism by GABA-T is presented. The micromethod involves the electrophoresis, in 1 mm diam. capillaries, of small samples of mixtures of [14C]GABA and its metabolites. By coupling this procedure to previously devised micromethods, it was possible to evaluate GABA-T attack to 14C labeled GABA diffusing across a single microdissected neuronal membrane.

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