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. 2021 Sep:129:105195.
doi: 10.1016/j.archoralbio.2021.105195. Epub 2021 Jun 8.

Homeobox gene amplification and methylation in oral squamous cell carcinoma

Maria Fernanda Setúbal Destro Rodrigues  1 , Flávia Caló Aquino Xavier  2 , Carina Duarte Esteves  3 , Rebeca Barros Nascimento  2 , Juliana Stephan Nobile  1 , Patrícia Severino  4 , Rafael de Cicco  5 , Tatiana Natasha Toporcov  6 , Eloiza Helena Tajara  7 , Fábio Daumas Nunes  8
Affiliations

Homeobox gene amplification and methylation in oral squamous cell carcinoma

Maria Fernanda Setúbal Destro Rodrigues et al. Arch Oral Biol. 2021 Sep.

Abstract

Objectives: Investigate the DNA copy number and the methylation profile of the homeobox genes HOXA5, HOXA7, HOXA9, HOXB5, HOXB13, HOXC12, HOXC13, HOXD10, HOXD11, IRX4 and ZHX1, and correlate them with clinicopathological parameters and overall survival.

Material and methods: DNA from OSCC samples and surgical margins were submitted to DNA amplification by qPCR and to DNA methylation analysis using a DNA Methylation PCR Array System.

Results: HOXA5, HOXB5 and HOXD10 were amplified in surgical margins while HOXA9, HOXB13 and IRX4 were amplified in OSCC. HOXD10 demonstrated hypermethylation in half of the tumor while ZHX1 did not show hypermethylation. No correlation of DNA copy number or methylation with clinicopathological parameters or survival was observed.

Conclusion: HOXA9, HOXB13 and IRX4 genes appears to be regulated by amplification and HOXD10 by methylation in OSCC. Further studies are needed to determine the role of these events in OSCC development.

Keywords: DNA methylation; Gene amplification; Homeobox genes; Oral squamous cell carcinoma.

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