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. 1988 Aug 11;16(15):7563-82.
doi: 10.1093/nar/16.15.7563.

Optimized conditions for pulsed field gel electrophoretic separations of DNA

B W Birren  1 , E Lai, S M Clark, L Hood, M I Simon
Affiliations
Free PMC article

Optimized conditions for pulsed field gel electrophoretic separations of DNA

B W Birren et al. Nucleic Acids Res. .
Free PMC article

Abstract

Quantitative measurement of DNA migration in gel electrophoresis requires precisely controlled homogeneous electric fields. A new electrophoresis system has allowed us to explore several parameters governing DNA migration during homogeneous field pulsed field gel (PFG) electrophoresis. Migration was measured at different switch times, temperatures, agarose concentrations, and voltage gradients. Conditions which increase DNA velocities permit separation over a wider size range, but reduce resolution. We have also varied the angle between the alternating electric fields. Reorientation angles between 105 degrees and 165 degrees give equivalent resolution, despite significant differences in DNA velocity. Separation of DNA fragments from 50 to greater than 7000 kilobases (Kb) can easily be optimized for speed and resolution based on conditions we describe.

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References

    1. Nucleic Acids Res. 1987 May 11;15(9):3930 - PubMed
    1. Cell. 1984 May;37(1):67-75 - PubMed
    1. Phys Rev Lett. 1987 Jun 8;58(23):2428-2431 - PubMed
    1. Science. 1986 Apr 4;232(4746):65-8 - PubMed
    1. Nucleic Acids Res. 1984 Jul 25;12(14):5647-64 - PubMed

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