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. 2021 May 29:30:e00630.
doi: 10.1016/j.btre.2021.e00630. eCollection 2021 Jun.

Yeasts isolated from a lotic continental environment in Brazil show potential to produce amylase, cellulase and protease

Jéssyca Ketterine Carvalho  1   2 , Andressa Alves Silva Panatta  1   3 , Maruhen Amir Datsch Silveira  4   5 , Christophe Tav  4   5 , Susana Johann  6 , Maria Luiza Fernandes Rodrigues  1 , Cleide Viviane Buzanello Martins  1
Affiliations

Yeasts isolated from a lotic continental environment in Brazil show potential to produce amylase, cellulase and protease

Jéssyca Ketterine Carvalho et al. Biotechnol Rep (Amst). .

Abstract

Yeasts have wide applicability in the industrial field, as in the production of enzymes used in biocatalysts. Biocatalysts are more efficient when compared to chemical catalysts, with emphasis on hydrolytic enzymes, such as amylase, cellulase and protease. Here we focused on prospecting yeasts, with a high capacity to synthesize hydrolytic enzymes, from a continental lotic ecosystem environment in Brazil. 75 yeasts were grown in Yeast Extract-Peptone-Dextrose (YPD) medium supplemented with antibacterial and their capacity for enzymatic production was tested in specific media. Accordingly, 64 yeasts showed enzyme production capacity. From those, six showed good enzyme indexes, 3 for amylase, 2 for cellulase and 1 for protease. All showed at least one hydrolytic enzyme activity for the tested enzymes (amylase, cellulase and protease), which suggested that the yeasts are metabolically active. By sequencing the 26S gene, we identified Naganishia diffluens and Apiotrichum mycotoxinivorans as the species with highest enzyme production activities. Those species showed potential for application as biological catalysts in the biotechnological scope, collaborating in a sustainable way for the development of industrial products.

Keywords: Amylase; Biotechnology; Cellulase; Enzyme industry application; Protease.

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Conflict of interest statement

The authors report no declarations of interest.

Figures

None
Graphical abstract
Fig. 1
Fig. 1
Geographical location of the water collection points in the Toledo River - PR. (Source: Google Earth).
Fig. 2
Fig. 2
Flowchart of the steps of the molecular analyzes carried out for the genetic identification of the yeasts selected as good producers of amylase, cellulase and protease.
Fig. 3
Fig. 3
Yeast quantity isolated from the Toledo River producing amylase, cellulase and/or protease. (A) Yeast quantity separated by produced enzyme. (B) Venn diagram of number of individuals producing one or more enzymes.
Fig. 4
Fig. 4
Phylogenetic analysis using the 26S ribosomal RNA sequence from our individuals vs related species.
See this image and copyright information in PMC

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