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. 2022 Mar;67(1):72-78.
doi: 10.1007/s11686-021-00433-0. Epub 2021 Jun 17.

Molecular Evidence and Hematological Profile of Bovines Naturally Infected with Ehrlichiosis in Southern Punjab, Pakistan

Muhammad Abdul Basit  1 , Muhammad Ijaz  2 , Jawaria Ali Khan  1 , Kamran Ashraf  3 , Rao Zahid Abbas  4
Affiliations

Molecular Evidence and Hematological Profile of Bovines Naturally Infected with Ehrlichiosis in Southern Punjab, Pakistan

Muhammad Abdul Basit et al. Acta Parasitol. 2022 Mar.

Abstract

Purpose: Ehrlichiosis is one of the tick-borne diseases, which is not only neglected in Pakistan but only a few reports have been documented throughout the globe. The purpose of this study is to highlight and report the neglected pathogen from bovines in Pakistan.

Methods: In this study, the pathogen was detected initially based on microscopy, followed by the molecular confirmation and phylogenetic analysis of the pathogen from bovines from south Punjab, Pakistan. The hematological parameters were also assessed in Ehrlichia positive and negative animals. The information of different disease determinants was analyzed by a logistic regression model on SPSS.

Results: The study has reported an 11.98% (23/192) prevalence of Ehrlichiosis from bovines. The prevalence was slightly more in cattle (13.5%) as compared to the buffaloes (10.4%). The previous tick's history and tick control methods were proved to be the key factors with the occurrence of the disease. The isolates from Pakistan Ehrlichia spp. Pakistan/31, 36, and 8 clustered with the isolates from the USA, Nicaraguan, France, South Africa, and Uganda. Platelet count, hemoglobin level, and hematocrit were found significantly decreased in Ehrlichia affected animals when compared to the healthy bovines.

Conclusion: This is the first report of ehrlichiosis from bovines in Pakistan and will provide the roadmap for future research.

Keywords: Bovines; Ehrlichiosis; Pakistan; Phylogenetic analysis; Risk factors.

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