Chitosan/PDLLA-PEG-PDLLA solution preparation by simple stirring and formation into a hydrogel at body temperature for whole wound healing

Abstract

Various chitosan (CS)-based dressings are used for wound treatment in clinical settings. Dressings that can be easily prepared and conveniently applied to treat wounds are highly desirable. In this study, a hybrid hydrogel was prepared using CS and poly (D,l-lactide)-poly (ethylene glycol)-poly (D,l-lactide) (PPP) through a simple process for convenient application. The optimal formula included a 7% CS PPP micellar solution, exhibiting excellent liquidity and allowing optional application as a spray. Molecular dynamic simulations indicated that CS and PPP established interactions via H-bonds and formed a long-chain complex, easily forming a hydrogel. Upon application, it rapidly transformed into a hydrogel and tightly adhered to the skin, forming a hemostatic (decreased 53.4%) and antibacterial (increased to about 90%) barrier, demonstrating accelerated wound healing (58.0%). This simple CS hybrid hydrogel can be easily prepared and applied and has potential applicability in clinical wound treatment.

Keywords: Chitosan; Hydrogel dressing; Wound healing.