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. 2021 Oct;77(10):4709-4718.
doi: 10.1002/ps.6513. Epub 2021 Jul 2.

Seed priming with calcium chloride enhances wheat resistance against wheat aphid Schizaphis graminum Rondani

Jun Wang  1   2   3 , Jia Song  1   2   3 , Xiao-Bao Wu  1   2   3 , Qian-Qian Deng  1   2   3 , Zhong-Yan Zhu  1   2   3 , Ming-Jian Ren  4 , Mao Ye  1   2   3 , Ren-Sen Zeng  5
Affiliations

Seed priming with calcium chloride enhances wheat resistance against wheat aphid Schizaphis graminum Rondani

Jun Wang et al. Pest Manag Sci. 2021 Oct.

Abstract

Background: Calcium is an essential macronutrient for plant growth. Although it has been shown that exogenous Ca application can increase plant resistance to abiotic stress, little is known about its potential to enhance plant tolerance to biotic stress. Here, we investigated whether pretreatment of wheat (Triticum aestivum L.) seeds with calcium chloride (CaCl2 ) improves plant resistance against wheat aphid (Schizaphis graminum Rondani). The developmental time, population size, feeding behavior of aphids on plants grown from CaCl2 - and water-pretreated seeds, and plant defense responses to aphid attack were investigated.

Results: Seed pretreatment with CaCl2 extended aphid development time and reduced aphid population size and feeding efficiency. In addition, the pretreatment significantly increased the concentration of Ca2+ in wheat leaves, and upregulated expression levels of TaCaM genes and callose synthase genes (TaGSL2, TaGSL8, TaGSL10, TaGSL12, TaGSL19, TaGSL22 and TaGSL23). Callose concentration in the leaves of plants grown from CaCl2 -pretreated seeds increased significantly upon aphid attack. Further, callose deposition was observed mainly in the phloem.

Conclusion: These results suggest that seed pretreatment with CaCl2 primes the plant response against wheat aphid attack, leading to modulation of callose deposition in the phloem in response to aphid attack. © 2021 Society of Chemical Industry.

Keywords: calcium; callose; herbivore resistance; seed priming; wheat (Triticum aestivum L.); wheat aphid (Schizaphis graminum Rondani).

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References

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