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. 2021 Sep;114(9):1339-1360.
doi: 10.1007/s10482-021-01604-9. Epub 2021 Jun 20.

Bacterial diversity and their metabolic profiles in the sedimentary environments of Ny-Ålesund, Arctic

Femi Anna Thomas  1   2 , Mahesh Mohan  3 , K P Krishnan  4
Affiliations

Bacterial diversity and their metabolic profiles in the sedimentary environments of Ny-Ålesund, Arctic

Femi Anna Thomas et al. Antonie Van Leeuwenhoek. 2021 Sep.

Abstract

Sedimentary environments in the Arctic are known to harbor diverse microbial communities playing a crucial role in the remineralization of organic matter and associated biogeochemical cycles. In this study, we used a combination of culture-dependent and culture-independent approaches to understanding the bacterial community composition associated with the sediments of a terrestrial versus fjord system in the Svalbard Arctic. Community-level metabolic profiling and growth response of retrieved bacterial isolates towards different carbon substrates at varying temperatures were also studied to assess the metabolic response of communities and isolates in the system. Bacterial species belonging to Cryobacterium and Psychrobacter dominated the terrestrial and fjord sediment retrievable fraction. Amplicon sequencing analysis revealed higher bacterial diversity in the terrestrial sediments (Shannon index; 8.135 and 7.935) as compared to the fjord sediments (4.5-5.37). Phylum Proteobacteria and Bacteroidetes dominated both terrestrial and fjord sediments. Phylum Verrucomicrobia and Cyanobacteria were abundant in terrestrial sediments while Epsilonbacteraeota and Fusobacteriia dominated the fjord sediments. Significant differences were observed in the carbon substrate utilization profiles between the terrestrial and fjord sediments at both 4 °C and 20 °C incubations (p < 0.005). Utilization of N-acetyl-D-glucosamine, D-mannitol and Tween-80 by the sediment communities and bacterial isolates from both systems, irrespective of their temperature incubations implies the affinity of bacteria for such substrates as energy sources and for their survival in cold environments. Our results suggest the ability of sediment bacterial communities to adjust their substrate utilization profiles according to condition changes in the ecosystems and are found to be less influenced by their phylogenetic relatedness.

Keywords: Amplicon sequencing; Community-level metabolic profiling; Culture-dependent; Fjord; Growth response; Terrestrial.

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