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. 2021 Mar;13(1):22-28.
doi: 10.1055/s-0041-1726569. Epub 2021 May 22.

Histomorphological and Morphometric Evaluation of Microvessel Density in Nodal Non-Hodgkin Lymphoma Using CD34 and CD105

Sucheta 1; Sant Prakash Kataria  1 , Shivani Malik  1 , Roomi Yadav  1 , Raman Kapil  1 , Rajeev Sen  1
Affiliations

Histomorphological and Morphometric Evaluation of Microvessel Density in Nodal Non-Hodgkin Lymphoma Using CD34 and CD105

Sucheta 1 et al. J Lab Physicians. 2021 Mar.

Abstract

Background Expression of angiogenic markers determined by microvessel density (MVD) could be used as a reliable predictor of prognosis and as a potential target for antiangiogenic therapy in different categories of non-Hodgkin lymphoma (NHL). Aims The aim of this study was to evaluate MVD using immunohistochemical methods and computer-assisted quantitative image analysis in nodal NHL patients and compare CD34 and CD105 expression in lymph nodes of NHL patients. Materials and Methods The present study was conducted on 60 lymph node biopsies received in the Department of Pathology at our tertiary care center for histopathological examination. Representative paraffin-embedded tissue sections were stained with hematoxylin and eosin along with immunohistochemical stains for CD34 and CD105. MVDs were analyzed at 400× using automated image analyzer by two investigators independently. Statistical Analysis Data were calculated, tabulated, and statistically analyzed using SPSS (Statistical Package for Social Studies) statistical program version 18. The values entered were mean of morphometric parameters. In all tests, p -values below 0.05 were regarded as significant. Results MVD was determined by CD34 and CD105 antibody highly correlated with different categories of NHL. Higher MVD was observed in cases of aggressive NHL as compared with indolent NHL and the difference was statistically significantly. MVD using CD105 was correlated more strongly as compared to CD34 with different categories of NHL. Conclusion The present study concluded that NHL exhibits potent angiogenic activity that increased significantly with increasing aggressiveness. The study also demonstrated that CD105 is more specific than CD34 as a marker of neoangiogenesis in NHL.

Keywords: CD105; angiogenesis; microvessel density; non-Hodgkin lymphoma.

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Conflict of interest statement

Conflicts of InterestFinancial Support and SponsorshipContribution of AuthorsNote The authors have no conflicts of interest to declare. None. Sucheta was involved in designing, definition of intellectual content, literature search, data acquisition, data analysis, and manuscript preparation. Sant Prakash Kataria did conceptualization, data analysis, manuscript editing, and manuscript review. Shivani Malik was involved in statistical analysis. Roomi Yadav did literature search. Raman Kapil did data analysis and literature search. Rajeev Sen did conceptualization and editing. The manuscript has been read and approved by all the authors and the requirements for authorship have been met. Each author believes that the manuscript represents honest work.

Figures

Fig. 1
Fig. 1
Comparison of mean MVDs using CD34 and CD105 antibodies in Group I (RL) and Group II (NHL). MVD, microvessel density; NHL, non-Hodgkin lymphoma; RL, reactive lymphadenitis.
Fig. 2
Fig. 2
Microvessels in reactive lymphadenitis (CD34; 400×).
Fig. 3
Fig. 3
Microvessels in reactive lymphadenitis (CD105; 400×).
Fig. 4
Fig. 4
Microvessels in peripheral T cell lymphoma (CD34; 400×).
Fig. 5
Fig. 5
Microvessels in peripheral T cell lymphoma (CD105; 400×).
See this image and copyright information in PMC

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