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Review
. 2021 Jun 4:8:686088.
doi: 10.3389/fvets.2021.686088. eCollection 2021.

Use of Precision-Cut Tissue Slices as a Translational Model to Study Host-Pathogen Interaction

Dominika Majorova  1 , Elizabeth Atkins  2 , Henny Martineau  1 , Ivan Vokral  3 , Dorenda Oosterhuis  4 , Peter Olinga  4 , Brendan Wren  2 , Jon Cuccui  2 , Dirk Werling  1
Affiliations
Review

Use of Precision-Cut Tissue Slices as a Translational Model to Study Host-Pathogen Interaction

Dominika Majorova et al. Front Vet Sci. .

Abstract

The recent increase in new technologies to analyze host-pathogen interaction has fostered a race to develop new methodologies to assess these not only on the cellular level, but also on the tissue level. Due to mouse-other mammal differences, there is a desperate need to develop relevant tissue models that can more closely recapitulate the host tissue during disease and repair. Whereas organoids and organs-on-a-chip technologies have their benefits, they still cannot provide the cellular and structural complexity of the host tissue. Here, precision cut tissue slices (PCTS) may provide invaluable models for complex ex-vivo generated tissues to assess host-pathogen interaction as well as potential vaccine responses in a "whole organ" manner. In this mini review, we discuss the current literature regarding PCTS in veterinary species and advocate that PCTS represent remarkable tools to further close the gap between target identification, subsequent translation of results into clinical studies, and thus opening avenues for future precision medicine approaches.

Keywords: host-pathogen interaction; immunology and infectious diseases; precision cut tissue slices; vaccinology; veterinary.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Summary of procedure and analyses possible to perform with precision cut tissue slices. Here, the process for lung slices is shown. Created with BioRender.com.
See this image and copyright information in PMC

References

    1. Alsafadi HN, Uhl FE, Pineda RH, Bailey KE, Rojas M, Wagner DE, et al. . Applications and approaches for three-dimensional precision-cut lung slices. Disease modeling and drug discovery. Am J Respir Cell Mol Biol. (2020) 62:681–91. 10.1165/rcmb.2019-0276TR - DOI - PMC - PubMed
    1. Bryson KJ, Garrido D, Esposito M, McLachlan G, Digard P, Schouler C, et al. . Precision cut lung slices: a novel versatile tool to examine host-pathogen interaction in the chicken lung. Vet Res. (2020) 51:2. 10.1186/s13567-019-0733-0 - DOI - PMC - PubMed
    1. Ebsen M, Mogilevski G, Anhenn O, Maiworm V, Theegarten D, Schwarze J, et al. . Infection of murine precision cut lung slices (PCLS) with respiratory syncytial virus (RSV) and chlamydophila pneumoniae using the Krumdieck technique. Pathol Res Pract. (2002) 198:747–53. 10.1078/0344-0338-00331 - DOI - PubMed
    1. Goris K, Uhlenbruck S, Schwegmann-Wessels C, Kohl W, Niedorf F, Stern M, et al. . Differential sensitivity of differentiated epithelial cells to respiratory viruses reveals different viral strategies of host infection. J Virol. (2009) 83:1962–8. 10.1128/JVI.01271-08 - DOI - PMC - PubMed
    1. Kirchhoff J, Uhlenbruck S, Goris K, Keil GM, Herrler G. Three viruses of the bovine respiratory disease complex apply different strategies to initiate infection. Vet Res. (2014) 45:20. 10.1186/1297-9716-45-20 - DOI - PMC - PubMed