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. 2021 May 31:2021:3439242.
doi: 10.1155/2021/3439242. eCollection 2021.

Extraction of Pregabalin in Urine Samples Using a Sulfonated Poly(ether ether ketone) Membrane

Chanbasha Basheer  1   2
Affiliations

Extraction of Pregabalin in Urine Samples Using a Sulfonated Poly(ether ether ketone) Membrane

Chanbasha Basheer. Int J Anal Chem. .

Abstract

In this work, a simple polymer-assisted microextraction technique was developed to determine pregabalin (an anticonvulsant drug) in the urine sample. A sulfonated poly(ether ether ketone) membrane was used as a sorbent for pregabalin extraction, and the extraction performance was compared with that of the conventional polydimethylsiloxane membrane. The extraction device is free moving and tumbles continuously throughout the stirred sample solution during extraction to enhance the extraction efficiency. The electrostatic interactions between the sulfonic-acid-functionalized polymeric membrane and the amine group in the pregabalin molecule facilitate higher preconcentration factor at a shorter extraction time. Optimizing conditions of the extraction method were investigated to obtain higher extraction efficiency. The developed method exhibited good linearity in the range of 0.05 to 2 µg/mL with a correlation of determination (r 2) 0.9998, acceptable limits of detection, limits of quantification, and preconcentration factor of 105-fold. The within-day and between-day precisions of pregabalin were lower than 7% relative standard deviations. Pregabalin was extracted from urine samples with recoveries of >92%, and no significant matrix effects were observed.

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Conflict of interest statement

The author declares that there are no conflicts of interest regarding the publication of this paper.

Figures

Figure 1
Figure 1
Chemical structure of (a) pregabalin and (b) SPEE.
Figure 2
Figure 2
Illustration for the ion-exchange mechanism and the electrostatic interactions between the SPEEK membrane and pregabalin analyte.
Figure 3
Figure 3
Comparison of SPEEK and PDMS as a sorbent. Extractions were performed at identical conditions.
Figure 4
Figure 4
Extraction profile of pregabalin using PME.
Figure 5
Figure 5
HPLC chromatogram of pregabalin (a) before extraction at 5 µL spiked sample and (b) after SPEEK extraction of drug-free urine sample spiked at 0.1 µg/mL.
See this image and copyright information in PMC

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