Biomass and salt-dependent effects of Bacillus spores on radionuclide migration from the Waste Isolation Pilot Plant

Abstract

Spores of a Bacillus sp., isolated from radioactive waste, were tested for their ability to influence the fate and transport of neodymium (Nd³⁺) under high salt conditions expected at the Waste Isolation Pilot Plant (WIPP) nuclear waste repository. Spores were suspended in neodymium-spiked saline solutions up to 4 M NaCl, and concentrations of Nd and the complexing agent dipicolinic acid (DPA), a component of spores, were monitored along with optical densities and spore numbers. Results support neodymium bioassociation that is dependent upon biomass, with more apparent adsorption occurring at higher spore concentrations. However, probable spore lysis in 2 and 4 M NaCl solutions and possible germination at 0.15 M NaCl appear to drive the release of DPA and subsequent return of Nd to solution. The implications of this work for the WIPP will depend on actual biomass levels and the ionic strength of the repository brines.

Keywords: Bacillus spores; Biological influence; Dipicolinic acid; Neodymium; WIPP.