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. 2021 Jun;1(6):e184.
doi: 10.1002/cpz1.184.

High-Dimensional Image Analysis using Histocytometry

Luis Munoz-Erazo  1 , Alfonso J Schmidt  1 , Kylie M Price  1
Affiliations

High-Dimensional Image Analysis using Histocytometry

Luis Munoz-Erazo et al. Curr Protoc. 2021 Jun.

Abstract

Histocytometry is a technique for processing multiparameter microscopy images using computational approaches to identify and quantify cellular phenotypes. It allows for spatial analyses of cellular phenotypes in relation to each other and within defined spatial regions. The benefit of this technique over manual annotation and characterization of cells is a high degree of automation/throughput, significantly decreased user bias, and increased reproducibility. Recently, an increase in freely available software amenable to or deliberately designed for histocytometry has resulted in these complex analyses being available to a broader base of users who have amassed multi-component microscopic imaging data. This article provides an overview of a histocytometry pipeline, focusing on the strategic planning and software requirements to allow readers to perform cell segmentation, phenotyping, and spatial analyses to advance their research outputs. © 2021 Wiley Periodicals LLC.

Keywords: cell segmentation; cytometry; histocytometry; image analysis; immunophenotyping; microscopy.

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References

LITERATURE CITED

    1. Ali, H. R., Jackson, H. W., Zanotelli, V. R. T., Danenberg, E., Fischer, J. R., Bardwell, H., … Bodenmiller, B. (2020). Imaging mass cytometry and multiplatform genomics define the phenogenomic landscape of breast cancer. Nature Cancer, 1(2), 163-175. doi: 10.1038/s43018-020-0026-6.
    1. Amodio, D., Cotugno, N., Macchiarulo, G., Rocca, S., Dimopoulos, Y., Castrucci, M. R., … Petrovas, C. (2018). Quantitative multiplexed imaging analysis reveals a strong association between immunogen-specific B cell responses and tonsillar germinal center immune dynamics in children after influenza vaccination. Journal of Immunology, 200(2), 538-550. doi: 10.4049/jimmunol.1701312.
    1. Angelo, M., Bendall, S. C., Finck, R., Hale, M. B., Hitzman, C., Borowsky, A. D., … Nolan, G. P. (2014). Multiplexed ion beam imaging of human breast tumors. Nature Medicine, 20(4), 436-442. doi: 10.1038/nm.3488.
    1. Bodenmiller, B. (2016). Multiplexed epitope-based tissue imaging for discovery and healthcare applications. Cell Systems, 2(4), 225-238. doi: 10.1016/j.cels.2016.03.008.
    1. Costantini, A., Mancini, S., Giuliodoro, S., Butini, L., Regnery, C. M., Silvestri, G., & Montroni, M. (2003). Effects of cryopreservation on lymphocyte immunophenotype and function. Journal of Immunology Methods, 278(1-2), 145-155. doi: 10.1016/s0022-1759(03)00202-3.