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Comment
. 2021 Jul;141(7):1627-1629.
doi: 10.1016/j.jid.2021.01.002.

The Three Rs of Single-Cell RNA Sequencing: Reuse, Refine, and Resource

Quan M Phan  1 , Iwona M Driskell  1 , Ryan R Driskell  2
Affiliations
Comment

The Three Rs of Single-Cell RNA Sequencing: Reuse, Refine, and Resource

Quan M Phan et al. J Invest Dermatol. 2021 Jul.

Abstract

Single-cell RNA sequencing (scRNA-seq) provides an unprecedented ability to investigate cellular heterogeneity in entire organs and tissues, including human skin. Ascensión et al. (2020) combined and reanalyzed human skin scRNA-seq datasets to uncover new insights into fibroblast heterogeneity. This work demonstrates that new discoveries can be made from published data on the basis of principles of these three Rs: Reuse, Refine, and Resource.

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Conflict of interest statement

CONFLICT OF INTEREST

RRD is a consultant for AgeX, but this is not relevant to the content of this manuscript. The remaining authors state no conflict of interest.

Figures

Figure 1.
Figure 1.. Schematic presentation of the three Rs principle in scRNA-seq.
Previously published scRNA-seq data are used to generate the initial discoveries. Raw and processed data could be stored and queried using web-based resources. Datasets can be obtained and reused. The reanalysis of published data using refined approaches could yield new and important insights to further advance the field. Processed data can subsequently be stored through web-based resources to allow for easy access. scRNA-seq, single-cell RNA sequencing; UMAP, Uniform Manifold Approximation and Projection.
See this image and copyright information in PMC

Comment on

References

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