Genes with specific functions in the ovarian follicles of Calliphora erythrocephala (Diptera)

Abstract

Working with the large dipteran Calliphora erythrocephala, we have performed differential screening to identify genes actively expressed in the previtellogenic and early vitellogenic stages of oogenesis but silent during the early stages of embryogenesis. Clones containing sequences homologous to four such genes have been characterized. Two clones are homologous to the yolk protein 1 gene of Drosophila melanogaster. These two clones are expressed not only in the columnar follicle cells surrounding the oocyte but also in the border cells--a highly specialized subgroup of the follicle cells. This indicates a new function for these cells though previously to contribute mainly in the formation of the micropyle. A third clone, which is related to the D. melanogaster vitelline membrane protein genes of the cluster at chromosomal locus 26A, is expressed only in the perioocyte follicle cells and not the border cell population. The fourth clone encodes a sequence of unknown function which is abundantly expressed in the germ line cells of the follicle. Transcripts homologous to this clone persist into the mature follicle and initially appear concentrated at the anterior pole of the oocyte. The distribution of repetitious DNA within these four clones indicates that the C. erythrocephala genome has a short interspersion arrangement of repetitive DNA.