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Comparative Study
. 2021 Oct;101(2):115434.
doi: 10.1016/j.diagmicrobio.2021.115434. Epub 2021 May 21.

Evaluation of Three Immunoassays for the Rapid Detection of SARS-CoV-2 antigens

Ilaria Baccani  1 , Fabio Morecchiato  1 , Chiara Chilleri  2 , Chiara Cervini  2 , Emanuele Gori  3 , Daniela Matarrese  4 , Andrea Bassetti  5 , Manuela Bonizzoli  6 , Jessica Mencarini  7 , Alberto Antonelli  8 , Gian Maria Rossolini  9
Affiliations
Comparative Study

Evaluation of Three Immunoassays for the Rapid Detection of SARS-CoV-2 antigens

Ilaria Baccani et al. Diagn Microbiol Infect Dis. 2021 Oct.

Abstract

Three assays for SARS-CoV-2 antigen detection in nasopharyngeal swabs (Lumipulse® G SARS-CoV-2 Ag [LPG], STANDARDTM F COVID-19 Ag FIA [STF] and AFIAS COVID-19 Ag [AFC] were evaluated. Compared to RT-PCR, LPG, AFC and STF showed a variable sensitivity (87.9%, 37.5%, and 35.7%, respectively) and an overall high specificity (> 95%).

Keywords: COVID-19; SARS-CoV-2; antigen; chemiluminescent assay; lateral flow immunofluorescence assay.

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Figures

Fig 1
Fig. 1
Number of positive isolates detected by Lumipulse® G SARS-CoV-2 Ag, AFIAS COVID 19 Ag and STANDARDTM F COVID-19 Ag FIA correlated to the N gene Ct value by RT-PCR. Samples with Ct≥ 40 have been considered as negative.
See this image and copyright information in PMC

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