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. 2021 Jun 27;7(1):41.
doi: 10.1186/s40813-021-00220-3.

Effectiveness of two intramuscular combined vaccines for the control of Mycoplasma hyopneumoniae and porcine circovirus type 2 in growing pigs: a randomized field trial

Gwenaël Boulbria  1   2 , Sophie Brilland  3 , Charlotte Teixeira-Costa  4 , Mathieu Brissonnier  4 , Mathieu Charles  3 , Nathalie Capdevielle  3 , Valérie Normand  4   5 , Franck Bouchet  4   5 , Pauline Berton  4 , Roman Krejci  3 , Arnaud Lebret  4   5
Affiliations

Effectiveness of two intramuscular combined vaccines for the control of Mycoplasma hyopneumoniae and porcine circovirus type 2 in growing pigs: a randomized field trial

Gwenaël Boulbria et al. Porcine Health Manag. .

Abstract

Background: Mycoplasma hyopneumoniae and Porcine circovirus type 2 are two economically important pathogens affecting growing pigs. Control and prevention of both diseases can be accomplished by vaccination, together with biosecurity and good management practices. Many commercial vaccines are available. The aim of this study was to assess the efficacy of Hyogen® and Circovac® administered mixed at weaning and to compare this protocol with a competitor ready-to-use (RTU) vaccine.

Case presentation: A randomised field trial was designed in a commercial farrow-to-finish farm located in France. A total of 641 pigs born from 54 different sows were included in this study. Piglets at weaning were allocated into three groups: the first one vaccinated with Hyogen® and Circovac® combined (group A), the second one vaccinated with a competitor RTU vaccine (group B) and the last one unvaccinated. Only minor local reactions for both vaccination groups could be observed which revealed a good safety of both protocols. Both vaccination schemes in this trial didn't improve wean-to-slaughter growth performances but significantly reduced lung lesions, lung fissures and pleurisy at slaughter, produced a seroconversion for both M. hyopneumoniae and PCV-2 and significantly reduced the PCV-2 viral load in blood. When we compared groups A and B, we observed no significant differences in growth performances, mortality, clinical signs, percentages of affected lungs at slaughter, lung fissures and pleurisy, and no difference in pathogens detection. However, two statistical differences were observed between both vaccines: the mean lung lesion score and the percentage of extensive lung lesions were lower in group A. This is consistent with lower M. hyopneumoniae loads in the lower respiratory tract in pigs from group A but this difference was not statistically significant.

Conclusions: Results reported in this case study must be considered with caution since it was done in only one farm. In this trial, Hyogen® and Circovac® mixed together under field conditions offered a successful protection of growing pigs and significantly decreased the extension of lung lesions during a natural field challenge when compared with a competitor RTU vaccine.

Keywords: Mycoplasma hyopneumoniae; Porcine circovirus 2; Serology; Vaccine; qPCR.

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Conflict of interest statement

SB, MC, NC and RK are employed by Ceva Animal Health France (10, avenue de la Ballastière, 33500 Libourne, France) and were not involved in analysis and interpretation of the data. SB, NC and RK assisted AL and GB for the study design. SB and MC provided assistance for samples and data collection.

Figures

Fig. 1
Fig. 1
Average respiratory disease score (RDS) during the trial in group A and group B
Fig. 2
Fig. 2
M. hyopneumoniae qPCR results expressed in percentage of positive tracheobronchial swabs per treatment group and sampling point
Fig. 3
Fig. 3
Median M. hyopneumoniae copies/ml in qPCR positive samples (expressed in logarithmic scale) per treatment group and sampling point
Fig. 4
Fig. 4
Percentage of M. hyopneumoniae seropositive pigs per treatment group and sampling age using Idexx HerdChek M. hyopneumoniae ELISA. In this graph, seropositivity is defined as S:P ratio > 0.4
Fig. 5
Fig. 5
Percentage of M. hyopneumoniae pigs with S:P ratio > 1.5 per treatment group and sampling age using Idexx HerdChek M. hyopneumoniae ELISA
Fig. 6
Fig. 6
Mean (±SD) S:P values per treatment group and sampling age using Idexx HerdChek M. hyopneumoniae ELISA
Fig. 7
Fig. 7
Mean (±SD) PCV-2 S:P ratio ELISA values per treatment group and sampling age using Ingezim Circo IgG 11. PCV.K1 kit
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