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. 1988 Sep;107(3):1011-24.
doi: 10.1083/jcb.107.3.1011.

Fluorescent microtubules break up under illumination

G P Vigers  1 , M Coue, J R McIntosh
Affiliations

Fluorescent microtubules break up under illumination

G P Vigers et al. J Cell Biol. 1988 Sep.

Abstract

We have synthesized three new fluorescent analogues of tubulin, using fluorescein or rhodamine groups attached to N-hydroxy-succinimidyl esters, and have partially characterized the properties of these analogues. We have also further characterized the tubulin derivatized with dichlorotriazinyl-aminofluorescein that has previously been used in this and other laboratories. Our results show that all four analogues assemble into microtubules which break up when exposed to light of the wavelengths that excite fluorescence. This sensitivity places severe constraints on the use of these analogues in studies of microtubule dynamics.

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