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Review
. 2021;15(3):116-126.
doi: 10.1007/s12281-021-00420-y. Epub 2021 Jun 23.

Candida auris: Diagnostic Challenges and Emerging Opportunities for the Clinical Microbiology Laboratory

C Keighley  1   2   3   4 , K Garnham  5 , S A J Harch  6   7 , M Robertson  8 , K Chaw  9 , J C Teng  10 , S C-A Chen  1   2
Affiliations
Review

Candida auris: Diagnostic Challenges and Emerging Opportunities for the Clinical Microbiology Laboratory

C Keighley et al. Curr Fungal Infect Rep. 2021.

Abstract

Purpose of review: This review summarises the epidemiology of Candida auris infection and describes contemporary and emerging diagnostic methods for detection and identification of C. auris.

Recent findings: A fifth C. auris clade has been described. Diagnostic accuracy has improved with development of selective/differential media for C. auris. Advances in spectral databases of matrix-associated laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) systems have reduced misidentification. Direct detection of C. auris in clinical specimens using real time PCR is increasingly used, as is whole genome sequencing (WGS) to track nosocomial spread and to study phylogenetic relationships and drug resistance.

Summary: C. auris is an important transmissible, nosocomial pathogen. The microbiological laboratory diagnostic capacity has extended beyond culture-based methods to include PCR and WGS. Microbiological techniques on the horizon include the use of MALDI-TOF MS for early echinocandin antifungal susceptibility testing (AST) and expansion of the versatile and information-rich WGS methods for outbreak investigation.

Keywords: Antifungal resistance; Antifungal susceptibility testing; Candida auris; Candidaemia; Fungal outbreak; Future diagnostics; Invasive candidiasis; Matrix-associated laser desorption time of flight (MALDI-ToF); Multidrug resistance (MDR); Nosocomial infection; Whole genome sequencing.

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Conflict of interest statement

Conflict of InterestKeighley C, Garnham K, Harch S A J, Robertson M, Chaw K and Teng JC declare that they have no conflict of interest. Chen SC-A reports untied educational grants from MSD Australia and F2G Ltd outside the submitted work.

Figures

Fig. 1
Fig. 1
Characteristics of C. auris on SAB and on chromogenic media. BrillianceTM Candida Agar (Oxoid, Basingstoke, UK), CHROMagarTM Candida Medium (Becton Dickinson, Heidelberg, Germany), CHROMIDR Candida medium (bioMérieux, Marcy l’Étoile, France), CHROMagarTM Candida Plus (CHROMagar, France). Images adapted from [–66]
See this image and copyright information in PMC

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