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. 2021 Apr 15;20(1):641-648.
doi: 10.1007/s40200-021-00794-3. eCollection 2021 Jun.

Hydro alcoholic green tea extract effect on high fat diet treated NMRI mice and 3T3L1 cells

Farnaz Banakar  1   2 , Azadeh Ebrahim-Habibi  2 , Mahsa Mohammad-Amoli  3 , Raheleh Kheirbakhsh  4 , Mahsa Sadeghi-Afjeh  5 , Shadab Shahriari  3 , Bagher Larijani  1
Affiliations

Hydro alcoholic green tea extract effect on high fat diet treated NMRI mice and 3T3L1 cells

Farnaz Banakar et al. J Diabetes Metab Disord. .

Abstract

Purpose: In order to counteract the obesity has epidemics, since current anti-obesity drugs effects remain limited, there is a need to provide new options. As a project aiming to assess potential anti obesity natural compounds, the effects of consumption of a minimal dose of green tea hydro alcoholic extract (GT) on adipocyte differentiation of 3T3L1 cell line were investigated.

Methods: Obesity was induced in female NMRI mice (which are less used overall) by the use of a high fat diet. Mice were divided into four groups of control (C), treated control (TC), obese (O) and treated obese (TO). TC and TO groups received 8 mg/Kg/day of GT for 8 weeks, and weighted weekly, after what biochemical and histological parameters were measured. GT was used at doses of 100,150 and 200 µg/ml on 3T3L1, and staining with Oil-red-O was done for estimation of fat droplet accumulation.

Results: Body weight was found to be affected significantly by GT. Blood glucose levels did not show significant changes between groups, while triglycerides levels of the O group was significantly higher than the C group, but the TO group showed no significant difference with the C group upon GT treatment. Liver and visceral fat tissues showed more normalized tissue and less fat accumulation in the TO group. TO and TC groups showed an ameliorated morphologic state of liver tissues. GT was also able to decrease fat droplet formation in a dose-dependent manner.

Conclusions: Adding a minimal amount of GT to the daily consumption may have preventive effects on fat accumulation in healthy subjects, while in obese cases, GT shows significant therapeutic effect.

Keywords: 3T3L1; Adipogenesis; Green tea; High fat‐diet; NMRI mouse; Obesity.

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Conflict of interest statement

Conflict of interestAll authors declare to have no conflict of Interests.

Figures

Fig. 1
Fig. 1
Liver tissue of different groups stained with Hematoxilin and Eosin (H&E).Magnification × 10. a Control, b Treated Control, c Obese, d Treated Obese
Fig. 2
Fig. 2
Visceral tissue in different groups stained with Hematoxilin and Eosin (H&E). Magnification × 10. a Control, b Treated Control, c Obese, d Treated Obese
Fig. 3
Fig. 3
Liver tissue in different groups stained with Sudan black B. Magnification× 40. a Control, b Treated Control, c Obese, d Treated Obese
Fig. 4
Fig. 4
Visceral fat tissue in different groups stained with Sudan black B. Magnification× 40. a Control, b Treated Control, c Obese, d Treated Obese
Fig. 5
Fig. 5
The Effect of different doses of GT on 3T3L1 stained with Oil-red-O. Magnification× 40. a Cell culture medium; b Induction medium with out GT; c GT concentration 50 µg/ml; d GT concentration 100 µg/ml; e GT concentration 150 µg/ml; f GT concentration 200 µg/ml
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