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. 2018 Mar 20;8(6):e2763.
doi: 10.21769/BioProtoc.2763.

Microbial Mutagenicity Assay: Ames Test

Urvashi Vijay  1 , Sonal Gupta  2 , Priyanka Mathur  1 , Prashanth Suravajhala  2 , Pradeep Bhatnagar  1
Affiliations

Microbial Mutagenicity Assay: Ames Test

Urvashi Vijay et al. Bio Protoc. .

Abstract

The Microbial mutagenicity Ames test is a bacterial bioassay accomplished in vitro to evaluate the mutagenicity of various environmental carcinogens and toxins. While Ames test is used to identify the revert mutations which are present in strains, it can also be used to detect the mutagenicity of environmental samples such as drugs, dyes, reagents, cosmetics, waste water, pesticides and other substances which are easily solubilized in a liquid suspension. We present the protocol for conducting Ames test in the laboratory.

Keywords: Carcinogenicity; Gene mutation; Mutagenicity; Revertants; Salmonella strains.

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Figures

Figure 1.
Figure 1.. Genetic approach for assessing the mutagenicity in Salmonella strains
(modified from https://en.wikipedia.org/wiki/Ames_test)
Figure 2.
Figure 2.. Spontaneous revertants colonies obtained after addition of waste water from health center in Salmonella mutagenicity assay at different concentrations, viz. 2 µl, 10 µl, 50 µl, 100 µl
(Vijay, 2014)
See this image and copyright information in PMC

References

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